| Background:Gastric cancer,one of the world's high incidence of malignant tumors,ranks fourth in all malignancies and ranks second in malignant tumor-related lethality.China has a high incidence of gastric cancer,the incidence was significantly higher than the world average.At present,the mechanism of gastric cancer development is not clear,affecting the further improvement of the diagnosis and treatment of gastric cancer.The aquaporin family(AQPs)belongs to the membrane intrinsic protein family,whose monomer size is about 30 kDa,and the main physiological functions are transmembrane passive transporting water molecules and small molecules such as glycerol and urea.Since the first discovery in 1988,the aquaporin family in the mammals have been found a total of 13 members:AQPO?AQP12.AQPs aredivided into three categories:(1)traditional aquaporins:selective water molecules,including AQP0,1,2,4,5,6,8.AQP6 and AQP8 are also involved in the transport of anions,ammonia and hydrogen peroxide(H2O2);(2)Glycerol channel proteins:transportable water molecules and uncharged small molecular solutes such as glycerol,Urea and other small molecule solutes,including AQP3,7,9,10;(3)non-traditional aquaporins:mainly found in the intracellular,with fewer sequence homology and infiltration function is unknown.These 13 proteins have different molecular weights,27kDa(AQP8)?37kDa(AQP7),and their expression in cells and tissues is spatially diverse,and the permeability of water molecules is different.AQP3 expression in a variety of tissues of the human body,especially in the digestive tract mucosal cells have a certain expression of abundance,which has become the impact of gastrointestinal diseases related to the development of the basis.Many in vivo and in vitro experiments have confirmed that AQP3 is associated with the proliferation of digestive tract tumors,and it can affect the invasion and metastasis of tumor cells through the corresponding signaling pathway.It has been reported that overexpression of AQP3 can promote the metastasis of colorectal adenocarcinoma cells.Objective:This study was aimed to investigate the effect of aquaporin 3(AQP3)on the proliferation and apoptosis of gastric cancer cells,and to study the possible molecular mechanisms.Methods:1.The expression of AQP3 in gastric cancer cell lines HGC27,MKN45,SGC7901,MGC803,BGC823,AGS and human gastric mucosal cell GES-1 were studied by RT-PCR and western-blot respectively from the transcription level and protein level.2.Construction of AQP3 low expression cell lines using small interfering RNA(siRNA)technique:SCG7901siAQP3,BGC823siAQP3.3.The proliferation of gastric cancer cells after AQP3 was detected by CCK8 cell proliferation assay.4.The apoptosis of gastric cancer cells after AQP3 was detected by flow cytometry.5.Autophagy levels were detected in AQP3 low expression cell lines and the number of autophagosomes was observed using confocal microscopy.Results:1.Compared with normal gastric mucosa cells,the expression of AQP3 in gastric cancer cell lines was significantly increased.2.The apoptosis level of gastric cancer cells after AQP3 was significantly increased.3.The proliferation of gastric cancer cells after AQP3 was significantly decreased.4.The level of autophagy in gastric cancer cells was significantly decreased after AQP3 interference,and the number of autophagosomes was significantly decreased.Conclusion:AQP3 is highly expressed in gastric cancer cells,and interfering with AQP3 can promote cell apoptosis by decreasing the level of autophagy and inhibit the proliferation of gastric cancer cells.It is revealed that AQP3 has a molecular target for gastric cancer. |
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