Study On The Mechanism Of The Effect Of Litchi Saponin On Diabetic Nephropathy

Objective:This research uses KM mice as the goal,diabetic nephropathy model induced by STZ,intragastric administration of saponin of Litchi(Saponin of Litchi Seed,SLD)and losartan intervention,intervention effect and mechanism of litchi saponin on diabetic nephropathy.Effect of losartan potassium and litchi seed saponin on blood glucose in DN mice;study of inflammatory cytokine interleukin-1 beta and interleukin-6 in DN play an important role in the development of;and effects of inflammatory cytokines MCP-1 and ICAM-1 in DN position.To provide experimental basis for clinical treatment of diabetic nephropathy.Method:KM mice were randomly divided into the normal control group and the model group.The KM mice were selected to prepare the model of diabetic nephropathy mice by intraperitoneal injection of STZ(150mg/kg).The successful preparation of the model,then randomly divided into model group,losartan group(10.0mg/Kg/d),saponin of Litchi Seed litchi saponin high dose group(10.0mg/Kg/d),dose of saponin of Litchi in Litchi saponin(5.0mg/Kg/d),low dose group(2.5mg/Kg/d),intragastric administration.Every week to detect blood glucose;gavage for 8 weeks after the detection of relative renal DN mice weight,blood biochemistry,renal pathological changes,by Western blot(Western blot)to observe the expression of MCP-1 nephropathy,diabetic kidney of mouse ICAM-1 protein.Result:1.blood glucose: compared with the normal control group,the fasting blood glucose(P<0.01)of the model group was significantly increased(KM),indicating that STZ can lead to a significant increase in blood glucose.2.relative kidney weight: compared with the normal healthy mice,the DN model mice kidney increased weight gain,there is a certain degree of renal injury.Compared with the model group,the intervention group showed lower relative kidney weight(P<0.01);the kidney of saponin of litchi and Losartan Potassium Tablets can reduce the DN mice,DN mice kidney has a protective effect to a certain extent.3.blood biochemistry.Compared with the normal control group,model group,IL-1 expression of mouse beta IL-6 in serum increased significantly(P<0.01);compared with the model group,each drug intervention group IL-1 beta and IL-6decreased significantly(P<0.01),litchi saponin in each dose group and the Losartan group showed no significant difference(P>0.05)reduced;That expression of inflammatory factor IL-1 beta and IL-6 in DN model in serum increased significantly,overexpression of saponin of Litchi in the three dose group and losartan can significantly inhibit the inflammatory cytokine IL-1 beta and IL-6 DN in sera of mice,inhibit the development of inflammation,delaying the development process of diabetic nephropathy.Renal pathology: compared with the model group,the normal control group,glomerular and tubular structure was normal,glomerular mesangial area without proliferation,capillary clear.In model group,glomerular hypertrophy,fibrosis,tubular epithelial cells and nuclear destruction,cavitation.Compared with the model group,SLD high dose group mice glomerular and renal tubular have obviously improved,SLD in low dose group,glomerular hypertrophy,capillary network structure collapse,balloon expansion of Zhang Xianming,foot process fusion was.4.MCP-1and ICAM-1: compared with normal control group,the expression of MCP-1 and ICAM-1 protein in renal tissue of model group mice was significantly increased(P<0.01);compared with the model group,each group of MCP-1 and ICAM-1 protein were significantly decreased(P < 0.01);litchi saponin high dose group and the Losartan group function is slightly different,but no statistical significance compared to SLD,medium and low dose group expression,but the difference did not mean Statistics(P>0.05).Conclusion:SLD can reduce the blood glucose of diabetic nephropathy mice,relative renal weight,improve the pathological morphology,has certain protective effect on diabetic nephropathy and its mechanism may be through inhibiting the expression of MCP-1 and ICAM-1 protein in kidney tissue development,reduce the content of inflammatory factor IL-1 beta and IL-6 in serum and inhibit and delay the diabetic kidney inflammation,to improve renal damage in diabetic nephropathy mice.