Experimental Study Of 3-bromopyruvate Inhibiting The Aerobic Glycolysis Of Hepatocarcinoma Cells HepG2

Objective To study the effect of 3-bromopyruvate(3-BrPA)on the aerobic glycolysis pathway of hepatocarcinoma cells HepG2.Methods The human hepatocarcinoma cells Hep G2 and the human normal liver cells QSG-7701 were treated with different concentration of 3-BrPA,the cell viability was detected by CCK8 assay.Morphological changes of the two cells were observed under microscope.The cell apoptosis rate was detected by flow cytometry.Real-timequantitative polymerase chain reaction(qPCR)and Western Blot were used for detecting the expression of c-Myc and MCT1 in HepG2 and QSG-7701 cells at mRNA and protein levels.The glucose uptake,lactate secretion and intracellular ATP of the cells were measured by enzyme-linked method.Results The cell survival rate and the cell apoptosis rate were significantly increased with the increase of 3-BrPA concentration(P<0.05).Compared with QSG-7701,the expression of c-Myc and MCT1 in HepG2 were significantly higher than those in QSG-7701 at mRNA and protein levels,and decreased with the increase of 3-BrPA concentration(P<0.05).The results of glucose metabolism analysis showed that the glucose uptake,lactate secretion and intracellular ATP of HepG2 decreased gradually with the increase of 3-BrPA concentration(P<0.05),but no significant change in QSG-7701(P>0.05).Conclusion 3-BrPA can inhibit the growth of hepatocarcinoma cells HepG2 and promote the cell apoptosis.The mechanism may be related to the inhibition of glycolysis by 3-BrPA and the expression of c-MYC,MCT-1,HK2,HepG2,PDK,PKM,LDHA,and the key enzymes involved in aerobic glycolysis in HepG2 cells.3-BrPA had no significant effect on normal liver cells QSG-7701.