Meta-analysis And Nested Case-control Study About The Association Between Gene KCNQ1 Polymorphism And T2DM

Part one:Meta-analysis about the association between gene KCNQ1 polymorphism and T2 DM.Objective: Comprehensive assessment the association between gene KCNQ1 polymorphism rs2237892?rs2237895 and type 2 diabetes mellitus.Methods: Collecting Chinese and English literatures about the association between gene KCNQ1 polymorphism rs2237892?rs2237895 with T2 DM in case-control and/or cohort study which are published before 2017 September 25 in Pub Med?SPRINGER?CNKI?WAN-FANG DATE?VIP database?China Biology medicine disc?China National Knowledge Infrastructure.OR and 95%CI of allele and genotype distribute frequency as combined statistics,STATA12.0 software heterogeneous test included literature results,funnel and Egger's regression tests evaluate publish bias of included literatures,forest figure estimate the correlation of gene KCNQ1 polymorphism and T2 DM.Results: 40 literatures about gene KCNQ1 polymorphism rs2237892 are included,compose of 61757 T2 DM patients and 59078 controls,distribute frequency of C allele of this site in T2 DM is higher than control,OR(95%CI)is 1.29(1.27,1.32),shows greatly statistical significance(z = 24.29,P < 0.001);21 literatures about rs2237985 are included,42594 T2 DM patients and 45306 controls are composed.distribute frequency of C allele of this site in T2 DM is higher than control,total OR(95%CI)is 1.24(1.22,1.27),shows outstanding statistical significance(z = 20.14,P < 0.001).Conclusions: Gene KCNQ1 polymorphism rs2237892?rs2237895 all associate with T2 DM morbidity.Part two: Nested case-control study about the association between gene KCNQ1 polymorphism and T2DMObjective: Investigation the relationship of gene KCNQ1 polymorphism rs2237892?rs2074196?rs151290 and T2 DM in Ganzhou Han population.Methods: Using nested case-control study to select 513 T2 DM patients and 502 NGT controllers without consanguinity,Matrix assisted laser desorption ionization time of flight(MALDI-TOF)mass spectrometry detect gene KCNQ1 polymorphism rs2237892?rs2074196?rs151290 in select,random 5% results samples are tested by direct sequencing;SPSS14.0 statistical software package is used to analysis the genotype results.Results:(1)There is no statistical significance of gender and age difference in T2 DM group and NGT group(P>0.05),FPG?Hb A1C?TG?TC?LDL-C in T2 DM group are all higher than NGT group,HDL-C is lower,the difference have statistical significance(P<0.05).(2)Distribute difference of genotype observation and excepted value do not show statistical significance in rs2237892?rs2074196?rs151290 of NGT group(x2=0.926?P=0.336,x2=0.0007?P=0.979,x2=0.089?P=0.764).This accords with Hardy-Weinberg law,these samples represent population.(3)rs2237892 site has three genotypes: CC?CT?TT,rs2074196 site has three genotypes: GG?GT?TT,rs151290 site has three genotypes: CC?CA?AA.Matrix assisted laser desorption ionization time of flight(MALDI-TOF)mass spectrometry results are consistent with direct sequencing test results.(4)The genotype frequency of rs2237892 site CC vs TT in T2 DM group is remarkable higher than NGT group,difference has statistical significance(x2=4.502,P=0.034),there are no statistical significance of CC vs CT genotype frequency in T2 DM and NGT group(x2=2.275,P=0.131),no statistical significance of CT vs TT in T2 DM and NGT group(x2=1.602,P=0.206),there are all no statistical significance of CC+CT vs TT(dominant model)and CC vs CT +TT(recessive model)in groups(P>0.05);C allele genotype frequency in T2DM(71.15%)is higher than NGT(66.50%),difference have statistical significance(x2=5.049,P=0.025);CC genotype carrier is 1.647 times riskier suffered from T2 DM than TT genotype carrier(OR=1.647,95%CI:1.036-2.620).Allele C carrier is 1.242 times riskier than T carrier(OR=1:242,95%CI:1.028-1.501).(5)The genotype frequency of rs2074196 site GG?GT?TT are 41.67%?48.61%?9.72% in T2 DM group,are 39.44%?46.68%?13.88% in NGT group,distribute difference has no statistical significance in these two groups(x2=4.178,P=0.124);The allele G,T frequency are 65.97%?34.03% in T2 DM group,are 62.78%?37.22% in NGT group,there are also no statistical significance of distribute difference in these two groups(x2=2.229,P=0.135);dominant model GG+GT vs TT in T2 DM group(928.6%)is higher than NGT group(620.3%),difference of two groups show statistical significance(x2=4.167,P=0.041),but gene model of GT vs TT?GG vs GT?GG vs GT +TT have no statistical significance(P=0.051~0.915);the risk suffered from T2 DM in both GG,GT genotype are 1.497 times than in TT genotype(OR=1.497,95%CI:1.014-2.209).(6)rs151290 site CC,CA,AA genotype frequency are 39.09%?49.01%?11.90% in T2 DM group,are 36.57%?47.27%?16.16% in NGT,distribute difference between these two groups has no statistical significance(x2=3.805,P=0.149);allele C,A distribute frequency are 36.41%?63.59% in T2 DM group,are 39.80%?60.20% in NGT group,also no statistical significance on distribute difference between these two groups(x2=2.433,P=0.119);using different gene model combination of rs151290 site to compare in two groups.It shows that no combination model demonstrate statistical significance(P>0.05).Conclusions:(1)Gene KCNQ1 polymorphism rs2237892 is associated with the T2 DM morbidity in Ganzhou han population,CC is susceptibility gene,C is susceptibility allele.(2)Gene KCNQ1 polymorphism rs2074196 is potential susceptibility site,GG+GT are susceptibility genotype.(3)Gene KCNQ1 polymorphism rs151290 do not connect with T2 DM morbidity in Ganzhou han population.