Synthesis And Characterization Of Mn3O4@Albumin Nanoparticles

Cancer has been one of the biggest risks to human health since 21^st century.Various methods for cancer diagnosis and therapy have been emerged with the development of nanotechnology.Furthermore,novel theranostic nanomedicines capable of precise cancer diagnosis and highly efficient therapy have attracted more attention from researchers.Recently,albumin has been developed as drug nanocarriers for cancer theranostics application due to its superior biocompatibility.In this study,Mn₃O₄@albumin nanoparticles with tunable particle sizes were synthesized using albumin as nanoreactor.A novel Mn₃O₄@albumin nanoparticle was achieved for both potent photothermal effect and significant MR?magnetic resonance?signals.These investigations are shown as follows:Chapter 1.This chapter is an overview introduction of MRI agents,photo-induced tumor therapy and theranostic nanomedicines.The basis and main contents of this dissertation are proposed.Chapter 2.Mn₃O₄ nanocrystals were synthesized using bovine serum albumin?BSA?as nanoreactor,exhibiting tunable particle sizes well regulated through reaction time?3.4±0.5 nm for 1 min,5.5±0.8 nm for 10 min,9.1±0.8 nm for 4 h?.Moreover,various synthesis methods have been tried to achieve Mn₃O₄@albumin with larger particle sizes and excellent photothermal effect.Finally,the optimized preparation conditions have been determined as following:10 mg/mL BSA,pH 12,25?,and 4 h for reaction.Chapter 3.In this section,XRD and XPS were applied to determine the chemical composition of the prepared nanoparticles.Under irradiation,Mn₃O₄@albumin exhibited concentration-dependent photothermal effect with a photothermal conversion efficiency of42.5%,which is superior to commercial AuNRs and ICG.Furthermore,Mn₃O₄@albumin possess ideal photostability and excellent chemical stability.Furthermore,Mn₃O₄@albumin displayed a high r₁ value with poteinal MR imaging property.Chapter 4.In vitro cell studies including cellular uptake and photo-cytotoxicity were investigated.Murine breast cancer cell 4T1 was used as the cancer cell model.Mn₃O₄@albumin exhibited time-dependent cellular uptake,obtaining the Mn amount of1.35?g/10⁶ cells after 24 h incubation.On the basis of efficient cellular uptake of Mn₃O₄@albumin,4T1 cells were suffered severe photo-cytotoxicity under irradiation,indicating IC₅₀ value of 0.17 mM.In summary,we fabricated Mn₃O₄@albumin theranostic nanoparticles through hollow albumin as the nanoreactor,and explored their applications in MR imaging and phototherapy.The albumin composite theranostic nanoparticles are promising for cancer theranostics.