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Synergic Photothermal And Chemotherapy Of Tumors Based On Functionalized Molybdenum Disulfide Nanosheet

Posted on:2018-09-18Degree:MasterType:Thesis
Country:ChinaCandidate:J XiongFull Text:PDF
GTID:2334330542956497Subject:Biology
Abstract/Summary:PDF Full Text Request
The development of nanomaterials provides a new opportunity for the treatment of cancer.Nanomaterials for tumor treatment is mainly used in two modes.Nanomaterials is applied as drug carriers to transport drug to the tumor site.The other is constructing multifunctional nanosystems through engineering modification,which can achieve synergistic therapeutic effect.Molybdenum disulfide(MoS2)nanosheet has attracted special attention with its unique advantages,which mainly are reflected in the following aspects:(1)MoS2 nanosheet can be directly used as drug carrier to adsorb traditional medicine molecules;(2)MoS2 nanosheet has strong absorption in NIR region,which can efficiently convert solar energy into heat energy.It is indicted that MoS2 nanosheet is an ideal material for photothermal treatment of tumor;(3)MoS2 nanosheet is a kind of materials with high biological safety.Combined with the above advantages,MoS2 nanosheet is expected to be used as the basic materials for multimodal treatment of tumor.In this paper,we developed two different drug loading nanosystem for chemotherapy-photothermal synergistic therapy based on the special properties of MoS2 nanosheet,following the two parts:1.Chemotherapy-photothermal synergistic therapy based on MoS2@DOX.We developed a simple pH response release system for chemotherapy-photothermal synergistic therapy based on MoS2 nanosheet,which can direct adsorb DOX with excellent photo thermal conversion properties.The successful synthesized MoS2 nanosheet had good dispersibility in deionized water with uniform size and the diameter was about 100 nm.Then MoS2 nanosheet adsorbed amount of DOX through the hydrophobic force.The mass ratio of DOX and MoS2 nanosheet was reached to 40%after optimization.In acidic environment of tumor,the hydrophobic force was destroyed,which made DOX fall down from MoS2 nanosheet.In the presence of NIR,MoS2 nanosheet could significantly rise local temperature in tumor cells,directly caused cell apoptosis.At the same time,the rised temperature accelerated the release of DOX from MoS2 nanosheet,which contributed to enhance the effect of chemotherapy.The results showed that the synergistic kill effect of MoS2@DOX to tumor cells under NIR was much better.The cell survival rate was decreased from 60%to 35%.2.Chemotherapy-photothermal synergistic therapy based on MoS2@ZIF-8-DOX.The development of multimodal therapy for tumors is an important strategy for clinical applications.Therefore,we have developed a new nanodrug delivery system for chemotherapy-photothermal synergistic therapy.On the basis of the previous wok,we introduced ZIF-8,a kind of biodegradable metal organic framework material,to further increase the drug loading and avoid the drug leakage during the transportation process.A simple one pot synthesis method was used to synthesize the MoS2@ZIF-8-DOX that MoS2 nanosheet and DOX were entrapped into ZIF-8 at the same time.The structure of composite system of MoS2@ZIF-8-DOX would gradually disintegrate after circulating to the tumor acidic environment as the ZIF-8 was degraded in acidic condition.DOX and MoS2 nanosheet were released.DOX was used to evaluate the effect of chemotherapy while MoS2 nanosheet could rapidly increase the local temperature in the cell to induce apoptosis.The results in vitro studies showed that the system had good biological safety.The kill effect of multimodal therapy was better than that of a certain treatment mode.The cell survival rate of chemotherapy-photothermal synergistic therapy was as low as 22.37%.Based on the advantages of MoS2@ZIF-8-DOX,we believe that the synergistic therapy induced by pH and NIR has a broad application prospect in the clinical research of cancer.
Keywords/Search Tags:Cancer, Synergic therapy, MoS2 nanosheet, Photothermal therapy, Chemotherapy
PDF Full Text Request
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