Preparation Of Tissue Engineering Scaffolds Based On Electrospinning Technology Using Autologous Fibrinogen

Objective: Exploring the method of using the electro-spinning technology to prepare biological scaffold material out of discarded blood;evaluating the biocompatibility of the scaffold;discussing the characteristic and feasibility of the scaffold material serving as tissue engineering material.Methods: Fibrinogen is purified out of the discarded surgical autologous blood after the procedures of separation,centrifugation,purification,etc.Then it is made into fiber film by way of the electro-spinning technology.Observe the gross morphologies of the scaffolds gained through different density and use scanning electron microscope to observe threedimensional structure.The characteristics of scaffolds were determined by measuring the rate of in vitro degradation,hydrophilicity,surface roughness,etc.It was cocultured with human bone marrow mesenchymal stem cells in vitro,the scaffold material was evaluated for its biocompatibility by measuring cell viability,cell proliferation rate.Through animal experiments to observe the repair of skin lesions.Results: Tissue engineering bio-scaffolds with self-discarded blood as a material had good film-forming properties and a clear fiber structure.Scanning electron microscopy showed that the fibers of electrospinning scaffolds with uniform diameter and uniform pore size in the 130 mg/ml group showed criss-crossing fiber structure.Cells grew well in the scaffold and secreted more cell matrix;contact angle test.Contact angle test of each group was less than 90°.After co-cultured with human bone marrow mesenchymal stem cells and scaffold materials,they differentiated into osteogenic and chondrogenic directions.The specific staining effect was obvious.Cell proliferation experiments showed that the cells showed an increasing trend with the increase of days;DAPI staining and Live/dead experiments proved that the scaffold materials had good biocompatibility.Conclusion: Discarded blood is a major hospital waste,and the disposal of discarded blood consumes a lot of resources.In this study,we successfully prepared three-dimensional structure-like materials from discarded blood using electrospinning within a short timeframe.The preliminary study results showed that this method and material had great development potential in the tissue engineering field,and the good biocompatibility and physical properties of the material may allow it to become an ideal choice for application for skin wounds and the induced membrane technique for bone defects.