Synthesis Of 13~1-dicyanomethylene(pyro) Pheophorbideand Pdy Antitumor Activity

Women's health killer,cervical cancer,has long been plagued by women,the clinical treatment of cervical cancer mostly to take a comprehensive treatment program,including surgery,chemotherapy,radiotherapy,but the overall effect is not very good,with photosensitizer and light System development With the continuous development of science,photodynamic therapy?PDT?in early cervical cancer and precancerous lesions have made great progress in early patients can even be cured,for patients with advanced,can significantly improve their clinical symptoms,Extended survival.Photodynamic therapy is the participation of oxygen,with a certain dose of light beam irradiation in the lesion to reach a sufficient concentration of photosensitizer,in the light irradiation conditions,the photosensitizer is activated to absorb a sufficient amount of photons,to transfer their energy to the reaction?Here refers to the cancerous tissue?,including the energy transfer and transfer,in the participation of oxygen molecules through a type,two types of photodynamic mechanism of action mechanism of apoptosis induced by cancer cell death or necrosis of cancer cells to achieve cure purpose.Photosensitizer is a key factor in photodynamic therapy,the study of the physicochemical properties of photosensitizer is essential,photosensitizer less than630nm penetration of human tissue depth of 0.5cm,the wavelength band near the700nm photosensitizer,can effectively penetrate the human body Tissue depth of0.8cm,900nm photosensitizer energy distribution in other forms more,there are defects,we can see the appropriate wavelength of photosensitizer is essential.In this paper,Methyl Pyropheophorbide a?MPPa?was used as the raw material to carry out conjugation range amplification on the basis of its unique 18?electron system,and a series of wavelengths The cyanide chlorophyll a product at about 700 nm.The structures were characterized by ¹H-NMR,¹³C-NMR and MS.The cytotoxicity of the drug was observed by cell phagocytosis assay.The cytotoxicity of the drug to cancer cells was detected by MTT assay.