Pathological Diagnosis Of Hepatocellular Carcinoma Based On Multiphoton Microscopy

Liver cancer is one of most common malignant tumors in the world,of which hepatocellular carcinoma is about 90%,it has threatened people's health seriously.It has been clinically proved that the pathological diagnosis is the key to reduce mortality.Based on two-photon excited fluorescence(TPEF)and second harmonic generation(SHG),multiphoton microscopy with the advantages of high resolution,deeper tissue penetration and minor phototoxicity,is suitable for label-free and real-time imaging of biological tissue.In this study,we first used SHG microscopy to image microstructure of well-differentiated and poorly differentiated hepatocellular carcinoma,and the diagnostic features such as collagen content,average fiber number and average fiber length are extracted.Then we in vivo investigate normal and abnormal rat livers by three-dimensional imaging,and the diagnostic features such as the ratio between elastin and collagen,metabolic state of the hepatocytes and collagen content,are extracted to distinguish the normal and abnormal(BDL)rat livers.Our results show that MPM has the ability to visualize liver disease and diagnose hepatocellular carcinoma,and quantify the various structural features.Therefore this technique is a powerful imaging tool for the diagnosis of liver cancer in the future.