An Experimental Study On The Effect Of Double Acid Etched Titanium Surface Modified With Calcium-containing Nanoflakes On The Behavior Of Osteoblasts

Implants-prosthetic treatments have been developed for over 50 years.Nowadays,titanium implants have been widely applied to clinical practice.Up to now,large numbers of patients with edentulous jaw or defect of dentition have received implants-prosthetic treatments which brought them the better lives.However,most of the commercial implants are imported.In addition,long osseointegration process of traditional oral implants was still necessary.In this study,double acid etching titanium surface modified with calcium-containing nanoflakes were prepared and its effects on osteoblast behaviors in vitro were evaluated.We hope that the results of this study would be a valuable reference for oral implant surface modification designs.Objective:To prepare the double acid etching titanium surface modified with calcium-containing nanoflakes and evaluate its effects on osteoblast behaviors in vitro.Methods:Through double acid-etching and hydrothermal treatment with a mixed solution of Ca(OH)2/H2O2,two different calcium-containing nano flakes(1h and 6 h treatment groups)were synthesized on the double acid etching titanium surfaces.The sandblasting with large grit and acid etching(SLA)titanium surface was used as the control group,and two double acid etching titanium surfaces modified with different calcium-containing nanoflakes were used as two experiment groups.The micro-topography and surface elemental compositions of different titanium surfaces were observed and analyzed.MC3T3-E1 cells were seeded on samples of three groups to investigate osteoblast behaviors on different titanium surfaces.Results:Two uniform nanoflake-like structures containing trace calcium were prepared on double acid etching titanium surfaces.Compared with the SLA titanium surface,two modified titanium surfaces significantly up-regulated the adhesion,proliferation,ALP activity and expressions of osteogenic-related genes of MC3T3-E1 cells,especially the 1 h treatment group.Conclusions:The double acid etching titanium surfaces modified with calcium-containing nanoflakes could promote the adhesion,proliferation and differentiation of MC3T3-E1 cells.