| Objective:CD4+CD25+ T regs is a subtype of CD4+T cells,It has the function of active regulation or inhibition of other immune cells,which can inhibit the immune recognition and destruction by inhibiting the host immune system.Transcription factor Foxp3 is a specific molecular marker of CD4+ CD25+T regs,which regulates the development and maintenance of CD4+CD25+T regs.CCL17 and CCL22 are members of the chemokine family,with a common chemokine receptor CCR4,The combination of CCL17 and CCL22 with CCR4 can not only induce the migration of tumor cells,but also promote the proliferation of tumor cells,thereby promoting the invasion and metastasis of tumor cells.This paper discusses the relationship between CD4+CD25+T regs?Foxp3 and CCL17,CCL22 in the pathogenesis of HNSCC.Materials and Methods:? We have collected 40 patients(experimental group 1)with head and neck squamous cell carcinoma who have been diagnosed or treated in Otorhinolaryngology and Head and Neck Surgery Department of our hospital,from 2014.01 to 2015.12.There are 39 males and 1 females,aged 41-79 years old.Hypopharyngeal squamous cell carcinoma in 15 cases and laryngeal squamous cell carcinoma in 25 cases.According to the AJCC 2010 pTNM staging,20 cases of stage? and ? are the early laryngeal carcinoma group,and the ?-? stage of 20 cases are the advanced laryngeal carcinoma.At the same time,10 healthy volunteers are enrolled as control group(control group 1).The peripheral blood 2ml has been detected by flow cytometry,and the ratio of CD4+CD25+/CD4+ and CD4+CD25+Fxop3+/CD4+ are calculated,respectively.SPSS 23 is used for statistical analysis.? 20 cases of HNSCC operation in the same Department of Otolaryngology,from 2013.01 to 2015.12 have been selected,All patients are primary or recurrence after treatment(chemotherapy,surgery).All male,aged between 49-75.12 cases of laryngeal squamous cell carcinoma,8 cases of carcinoma of the hypopharynx.According to AJCC 2010 pTNM staging,are III,IV phase.The primary tumor is taken as the experimental group(experimental group 2),and the adjacent normal tissues from the primary tumor 1-3cm are taken as control group(control group 2).CD4+/Foxp3 and CD25+/Foxp3 are detected by immunofluorescence,and CCL17 and CCL22 are detected by ELISA.Statistical analysis is performed by SPSS23.0.Results:1?CD4+CD25+T regs is highly expressed in head and neck tumors,compared with the control group(control group 1)(P=0.000).The positive rate of late stage and early head and neck tumors is statistically significant(P=0.039).There is significant difference between the early and late stage(P=0.032).The positive rate of Foxp3+ is higher in CD4+CD25+T regs positive cells than in control group(control group 1)(P=0.000).The positive rate of late stage and early head and neck tumors is statistically significant(P=0.039).There is a significant positive correlation between CD4+CD25+T regs and Foxp3(i=0.95)2?There is statistical significance in CD4+/Foxp3 and CD25+/Foxp3 in the experimental group(experimental group 2)and the control group(control group 2)(P=0.046 vs P=0.025).There is significant difference in CCL17 and CCL22 between the two groups(P=0.000 vs P=0.000).There is a positive correlation between CD25+ and CCL17,CCL22(r=0.595,0.720).Conclusion:CD4+CD25+ T regs and Foxp3 are highly expressed in the peripheral blood of patients with head and neck squamous cell carcinoma.Through the inhibition of the immune system in patients with squamous cell carcinoma of the head and neck,the development of head and neck squamous cell carcinoma are promoted.CD4+CD25+ T regs and CCL17?CCL22 played an important role in the pathogenesis of head and neck squamous cell carcinoma,both of which interacted with each other,and promoted the recurrence and metastasis of head and neck squamous cell carcinoma. |
PDF Full Text Request