Drug Resistance Mechanism Of GPR120 In Breast Cancer

Part?The expression relationship and the biological meaning of GPR120 andGPR120 and ABCG2 in breast tumor tissuesObjective:To investigate the expression of GPR120 protein in breast tumor tissues,and the relationship between GPR120,ABCG2 protein as well as the biological meaning.Method:We make a collection of 212 patients'specimens and detect the expressions of GPR120,ABCG2 protein,using IHC,and statistical analysis.Result:?1?GPR120 protein was mainly expressed in the cells cytoplasm and membrane of breast invasive ductal carcinoma.The expression of GPR120 protein in cells membranes and cytoplasm of breast invasive ductal carcinoma was apparently higher than that in benign breast tumor?P<0.001?;In cells membrane of breast invasive ductal carcinoma,the expression of GPR120 protein has correlation with tumor grade?P=0.009?;In cells cytoplasm of breast invasive ductal carcinoma,the HER-2 positive patients'expression of GPR120 protein is higher than that with negative HER-2 expression,and it is statistically significant?P=0.020?.?2?The expression of ABCG2 protein in the cytoplasm is rising as severity of breast cancer?P=0.001?.?3?In cells cytoplasm of breast invasive ductal carcinoma,there is in common between the expression of GPR120 and ABCG2protein?P=0.049,P<0.001?;Our study suggest that in tumor size great than 3cm patients'co-expression of GPR120,ABCG2 protein is higher than that tumor size small than3cm?P=0.037?.Concusions:In cells cytoplasm of breast invasive ductal carcinoma tissue,there is in common between the expression of GPR120 and ABCG2 protein.There may be coadjustment of GPR120 protein and ABCG2 protein,which is associated with drug resistance and proliferation in breast cancer,which necessary to investigate further.Part?The regulation mechanism of GPR120 in breast cancer cell involvesin the regulation of drug resistance and the relationship with ABCG2Objective:To study the regulation mechanism of GPR120 and drug resistance in breast cancer cell lines and its signal pathway,which provide a new target for the treatment of breast cancer.Method:Using Western blotting,FCM,CCK8 and RT-PCR,we studied the expression of ABCG2 protein after and before stimulate of GPR120,and antagonism effect of DOX therapy.Finally,we searched for possible signaling pathways that the regulation mechanism of GPR120 and ABCG2.Result:?1?TUG-891 can stimulate GPR120 specifically.After activation of GPR120,the Ca²⁺concentration increases in breast cancer cell MCF-7.?2?After activation of GPR120,the apoptosis rate of breast cancer cell MCF-7 was significantly down-regulated.After activation of GPR120,the effect of high concentration of DOX that inhibit the proliferation was down-regulated?P<0.05?.After activation of GPR120,the expression of ABCG2 protein and m RNA,GPR120 protein were up-regulated?P<0.001?.?3?With ERK1/2 pathway blocking,the downstream target molecule ABCG2 protein decreased,apoptosis rate increased at breast cancer cell MCF-7 after activation of GPR120.Concusions:GPR120 involved in regulating the expression of ABCG2 protein and mRNA in proliferation and apoptosis of MCF-7 cells after DOX treatment.ERK1/2pathway maybe play important role between GPR120 and ABCG2 in breast cancer.