The Impact Of Almotriptan Malate On The Behavior And Expression Of Fos Protein In Periaqueductal Gray Of Migraine Rats

Purpose:The animal model of vascular migraine was established by electrically stimulating the dura mater surrounding the superior sagittal sinus.The animals' behavioral changes before and after electrical stimulation were observed and the changes of Fos positive cells in periaqueductal gray(PAG)were calculated.The efficacy of almotriptan malate in headache control was compared with classic analgesic drug--acetaminophen and their effects on the transmission of nociceptive information in PAG are also studied.Method:30 migraine rats from successfully established migraine model were randomly divided into 3 groups: normal saline intervention group(model group A),almotriptan malate intervention group(model group B),acetaminophen intervention group(model group C),and 10 non-surgical non-intervention normal rats were set as blank control group(blank group D).Group A,B,C were given electrical stimulation(6 Hz frequency,voltage 3V and pulse width 0.25 ms)for 1 hour after administration.The behavioral changes were observed before and after the administration of the conscious rats in the groups including model groups and blank group.The behavioral indexes included the time of excessive grooming,the time of freezing behavior,the times of climbing cage and pain threshold changes.After the fifth day of electrical stimulation,the rats in the model groups and blank group were perfused and fixed.The frozen sections were made in the intracranial periaqueductal gray,and then we performed immunohistochemical staining of Fos protein.The positive cells were counted by Image J software.Result:1.Almotriptan malate and acetaminophen can shorten the excessive-grooming time of migraine rats,and the former is more significant than the latter.2.Almotriptan malate and acetaminophen can inhibit the freezing behavior of migraine rats,and there was no statistically significant difference between the two.3.The effect of electrical stimulation and drug intervention on the rats' behavior of climbing cage was not significant,and there was no significant difference among these groups.4.Almotriptan malate and acetaminophen can raise the pain threshold of rats,relieve pain symptoms,and the former is more effective than the latter.5.Almotriptan malate and acetaminophen could reduce the expression of Fos protein in the PAG of migraine rats,and there was no significant difference between the two drugs.Conclusion:1.The experimental rats showed a series of characteristic behavioral manifestations after electrical stimulation,suggesting that the migraine model established by electrical stimulation of the dura mater surrounding the superior sagittal sinus,which is based on trigeminal neurovascular theory,is reliable.2.Almotriptan malate and acetaminophen could attenuate the behavioral responses to electrical stimulation of the dura mater in a migraine model,and the effective time of almotriptan malate in relieving migraine symptoms was better than that of acetaminophen.3.Almotriptan malate and acetaminophen can reduce the increased expression of Fos protein within the PAG of rats following electrical stimulation of the dura mater surrounding the superior sagittal sinus,inhibit the transmission of pain information and relieve the symptoms of migraine.