Expression Of B7-H3 In Cancer-associated Fibroblasts And Primary Research Of Its Biological Function In Microenvironment Of Renal Cell Carcinoma

Objective:To determine and evaluate the expression of B7-H3 in cancer-associated fibroblasts(CAFs)of renal cell carcinoma(RCC).To construct B7-H3~-CAFs cell model and explore its biological significance of occurrence and development of RCC.Methods:(1)We collected tumor tissues of 10 patients,who underwent radical nephrectomy between June and August in 2016 in the First Affiliated Hospital ofSoochow University.The expression of B7-H3 in CAFs was evaluated by flow cytometry and immunofluorescence.(2)CAFs were grown in primary culture and purified then.(3)We constructed B7-H3~-CAFs and B7-H3~+CAFs cell models by using Lentivirus LV-CD276 and negative control virus CON145 to transfect CAFs respectively.(4)8 SPF grade Balb/C nude mice were randomly assigned to two groups,the mice of experimental group were taken subcutaneous injection of RCC A498(10~6/100?L)and B7-H3~-CAFs(4x10~6/100?L),the mice of control group were taken subcutaneous injection of RCC A498(10~6/100?L)and B7-H3~+CAFs(4x10~6/100?L),and subcutaneous tumor model of RCC was constructed finally.Results:(1)A group of B7-H3~+CAFs exists in RCC.(2)Purified CAFs from RCC were acquired successfully.(3)B7-H3~-CAFs and B7-H3~+CAFs cell models were constructed successfully.(4)Subcutaneous tumor model of RCC was constructed successfully.The tumor of experimental group was smaller than the control group,and the difference became more obvious as time went.Conclusion:In microenvironment of RCC,CAFs expressing B7-H3 plays an important role in occurrence and development.Silence the expression of B7-H3 in CAFs may have a potential value of targeted therapies in RCC patients.