Preparation Of Novel Mesoporous Organosilica Nanoparticles And Their Biomedical Applications

Mesoporous organosilica nanoparticles(MONs)with organic groups distributed in the framework,which possess rich uniform pores,specific surface area,good stability.The nanoparticles can be used for drug carrier,genes and enzymes,showing great advantages in the field of cancer therapy.This paper mainly incorporates organic groups of various biological active in the skeleton simultaneously,the obtained nanoparticles are good biocompatible and have broad application prospects in drug delivery.Based on the above research,this paper is divided into three parts:1.Uniform yolk–shell-structured triple-hybridized MONs have been successfully prepared via a CTAB-directed sol–gel process of multiple bridged silanes and a hydrothermal treatment procedure.The MONs simultaneously and homogeneously incorporated with ethane,thioether,and benzene groups in the frameworks and possess large surface area,ordered mesochannels,large pore volume and tunable diameter.The MONs show lowered cytotoxicity,negligible hemolytic activity,good histocompatibility,and high anti-cancer drug loading capacity.The thioether groups in the framework give them a GSH-responsive drug release performance and the DOX loaded MONs exhibit enhanced therapeutic efficacy against tumor cells.Meanwhile,the MONs can be functionalized with fluorescence dye,which makes them promising candidates for in vivo imaging.2.Double-shelled yolk-shell mesoporous organosilica nanoparticles with thioether-bridge framework inner shell and ethane-bridge framework was developed for loading two different types of drugs.The nanoparticles possess high degree of condensation and good biocompatibility and blood compatibility.At the same time,the double-shelled yolk-shell mesoporous organosilica nanoparticles can co-encapsulate and co-deliver doxorubicin and cisplatin simultaneously.The dual-drug delivery system showd better in vitro therapeutic effect than the single drug delivery system.In addition,Cyanine 5.5 can be covalented with the mesoporous organosilica nanoparticles by click chemistry.3.Ultrasmall MONs(12 nm)uniformly integrated with thioether groups through self-assembly of organosilica precursor hydrolysis and condensation with CTAB in alkaline water solution were synthesized for the first time.In order to control the size of the nanoparticle and enable the nanoparticles with good solubility,adding PEG silane coupled in the reaction process termination.The obtained ultrasmall MONs can be facilitately connected with near infrared dye by click chemistry.