Identification Of Long Non-coding RNAs Of Vlinc Class Involved In Response To Anti-cancer Drugs

Most of human genome(98%)can not encode proteins,yet it a up to 75% of our DNA is used to make non-coding RNAs.Long noncoding RNAs(lncRNAs)(non-coding RNAs transcripts >200 bases)have been found to play a crucial role in many important biological processes of higher organisms in recent years.They often function via regulation of other genes involved in multiple cellular functions.Abnormal expression or functioning of these transcripts can was linked to a variety of major diseases,including cancers.In the human transcriptome,vlincRNAs represent a class of lncRNAs that appear to have a prominent role but remain mostly uncharacterized so far.Yet,they offer a potential new direction of lncRNA research,especially in cancer and early where a subgroup of these transcripts was shown to function.Considering the vast numbers of un-explored lnc-and vlincRNAs,its almost certain that these transcripts hold the keys to the mysteries a cell.For example,recent studies have shown that lncRNAs can mediate the effects of anti-cancer drugs.Therefore,these transcripts can potentially tell us a lot about molecular mechanisms of actions anti-cancer drugs and and their effects on cells.This paper focuses on discovery of vlincRNAs potentially involved in response to and/or mechanisms of action of anti-cancer drugs.The major goals is to understand the function of vlincRNAs and also the drugs that could lead to better anti-cancer therapies.We used RNAseq on a single molecule sequencing platform to identify vlincRNAs whose expression significantly changed in response to treatment of human leukemia cell line K562 treated with various anticancer drugs.I specifically focused on Imatinib(Gleevec)and Selumetinib because Imatinib is a a well established anti-cancer drug and Selumetinib is a experimental one.I found a number of vlincRNAs whose expression changed in in response to these drugs.I have characterized KVUG1 ? KVUG2 and KVUG3 such vlincRNAs to confirm the reproducibly and further characterize the kinetics of their response to these drugs.Also,I provide potential functions of these vlincRNAs using systems biology analysis.