Expression And Significance Of COX-2 In Acute Myeloid Leukemia And Its Correlation With WT P53

Objective:To investigate expression of COX-2 and WT p53 in bone marrow cells of incipient and relapse acute myeloid leukemia patients,and analyze their expression characteristics and correlation between both and their correlation with clinical features of patients,to explore possible mechanism of AML recurrence.Methods:(1)Total 82 cases of bone marrow samples without p53 mutation were obtained from patients in department of hematology of Second Affiliated Hospital of Shanxi Medical University from March 2016 to September 2016.AML patients(with the exception of acute promyelocytic leukemia)were 61 cases,including 37 incipient patients and 24 relapsed patients.Normal control group patients were 21 cases(including 14 patients with iron deficiency anemia and 7 cases with primary immune thrornbocytopenic).(2)Quantitative real-time PCR(RT-q PCR)and Western blot analysis detected the m RNA and protein expression level of COX-2 and WT p53 of clinical specimens.(3)SPSS 22.0 software and Graph Pad Prism 5.0 were used for statistical analysis and drawing.Results:(1)RT-q PCR and Western boltting analysis showed that COX-2 in bone marrow of AML patients was over-expressed,and COX-2 expression in relapsed patients was significantly higher than that in incipient patients(P<0.05),while WT p53 expression was lower with disease progression(P<0.05).(2)COX-2 expression in incipient and relapsed patients was negatively correlated with WT p53 expression(P<0.05),while it in normal group had no correlation(P>0.05).(3)The trend of higher COX-2 expression and lower WT p53 expression existed in different FAB sub-types patients,but only in M5 patients was statistically significant(P<0.05).(4)Compared with low and medium risk group,COX-2 expression in high-risk group were significantly increased(P<0.05).(5)The expression of COX-2 and WT p53 in incipient and relapsed patients had no correlation with gender,age,the platelet count and hemoglobin concentration.In relapse group,COX-2 expression increased with the peripheral blood leukocyte count,the percentage of leukemia cells increased and abnormal karyotype,while WT p53 expression decreased(P<0.05).Conclusions:COX-2,as an inflammatory molecule of chronic non-resolving inflammation,was over-expressed in AML,leading to the development and progress of AML by inhibiting the WT p53 expression.Their expression was correlation with high-risk group,peripheral blood leukocyte count,leukemia cell percentage and abnormal karyotype.