Influence Of Adenosine-Induced Epigenetic Changes In Colorectal Cancer Cells

Objective: To investigate the influence of adenosine(ADO)-induced epigenetic changes in colorectal cancer cells.Methods: SW480 cells were subjected to no treatment,ADO with a final concentration of 3 mmol/L,or 5-iodotubercidin(ITU)with a final concentration of 4 ?mol/L for 72 h.The human Mut L homolog 1(hMLH1)gene methylation levels of the CpG islands were detected by bisulfite sequencing polymerase chain reaction;hMLH1 mRNA expression levels were detected by reverse transcription-polymerase chain reaction;hMLH1 protein,DNA(cytosine-5)-methyltransferase 1(DNMT1),and DNA(cytosine-5)-methyltransferase 3A(DNMT3A)expression levels were detected by western blotting;and apoptosis rates were detected by flow cytometry.Results: The hMLH1 promoter methylation levels were significantly lower in the treatment groups than in the control group(P<0.01).The mRNA expression levels increased dramatically(P<0.01).The hMLH1 protein expression levels were significantly higher in the treatment groups than in the control group,especially in the ITU group(P<0.01).The DNMT1 and DNMT3 a expression levels were significantly lower than in the control group,especially the ITU group(P<0.01).The apoptosis rates were significantly higher than the control group,especially the ITU group(P<0.01).