A Novel Electrochemical Immunosensor For The Detection Of Vangl1,a Potention Biomarker For Neural Tube Defect

Vangl1 is one of the core proteins in PCP signaling pathway.The aberrant expression of Vangl1 is highly correlated with dysontogenesis,especially for neural tube defects(NTDs).Therefore,the ultrasensitive detection of Vangl1 would provide a new approach for the specific early diagnostics in dysembryoplasia.However,no quantitative detection method is available currently.Herein,we describe the development of a new approach to fill this assay gap.In recent years,immunsensor has been widely used in the field of clinical diagnosis and biological sample analysis because of its easily operating,low cost,low detection limit and high sensitivity.It has become an ideal analytical technique for biomarker detection.The principle of the sensor is transferred the biological molecules(nucleic acids,antibodies,antigens,enzymes,etc.)into sound,light,electricity and other signals which could be measured and analyzed.In this paper,we construct the immunosensor from the preparation of nanocomposites and the choice of signal amplification strategy.Then,we studed and analyzed the analytical properties of the immunosensor.The contents and results are as follows:Firstly,The C60-AuPt were used in this step as a signal enhancer due to the high electrical conductivity highly electrocatalytic activity for the reduction of H2O2.C60 was introduced as a carrier due to its large specific area and numerous conjugate ? electrons that can combine with AuPt.To further amplify the electrochemical signal,reduced graphene oxide-tetraethylene pentamine(rGO-TEPA)and a derivative of 3,4,9,10-perylenetetracarboxylicdianhydride(PTC-NH2)were selected for modification of the electrode to provide more amino groups for the immobilization of antibodies and to enhance the conductivity.Under optimal condition,the prepared immunosensor exhibited a wide linear range from 0.1 pg /mL to 450 pg /mL and a low detection limit of 0.03 pg /mL.Moreover,the proposed method exhibited good stability and recovery,suggesting its potential for use in clinical research.Our study provides a meaningful experimental basis for rapid clinical molecular diagnosis of dysontogenesis such as NTDs.