Imbalance Between Proliferation And Apoptosis-related Impaired GPR30 Expression Is Involved In Preeclampsia

Objective : To investigate whether G-protein coupled receptor 30(GPR30)can affect preeclampsia(PE)by regulating cell proliferation and apoptosis.Method:10 cases of normal placental tissue and 10 cases of PE placental tissue were collected from Chongqing First Affiliated Hospital of Chongqing Medical University,Chongqing,China from February to August 2014.The levels of GPR30 and proliferation-related factor ki-67,apoptosis-related factor caspase-3 and caspase-7 in placental tissues were detected by immunohistochemical staining(IHC)and Western blotting(WB).PE model of placenta were established by hypoxia-reoxygenation.17β-estradiol(E2)and G-1 which are the agonist of GPR30 and G-15 which is the inhibitor of GPR30 were,respectively or synergistically,used in normal placenta or PE model.Then the expression of GPR30 and related cytokines and cell signal pathway factors were detected by WB.Result : Low GPR30 expression levels,more apoptosis,and less proliferation were associated with PE.Moreover in vitro studies have shown that both the selective GPR30 agonist G1 and E2 were able to prevent apoptosis and cell proliferation decrease caused by hypoxia and reoxygenation in placenta.And this protective effect was abolished by selective GPR30 inhibitor G15.Conclusion:(1)GPR30 is involved in regulating cell proliferation and apoptosis;(2)pharmacologic upregulation of GPR30 is beneficial for PE management;(3)GPR30 may therefore be an interventional target for pregnancies complicated by PE.