Biocompatibility Of Graphene\Nanohydroxyapatite\Polyamide 66 Composite Material

Objective:To evaluate the biocompatibility of graphene/nanohydroxyapatite/polyamide 66 composite material(G/n-HA/PA66).Methods:G/n-HA/PA66 composite was prepared by solution blending method.Material extract solution was extracted as specified by GB/T 16886.5-2003/ISO 10993-5:2009.The experiments were divided into 3 groups: experimental group,negative control group,and positive control group.The biocompatibilities of G/n-HA/PA66 composite were evaluated according to in vitro cytotoxicity test,acute systemic toxicity test,hemolysis test,pyrogen test and intradermal stimulation test.Results:In vitro cytotoxicity test showed that the cell morphology and proliferation had a good condition and there was a significant statistical difference between each experimental group and positive group(P=0.000).The cell cycle of C3H10T1/2 in G2/M and S period could not be affected by composite material(P=0.889).The cell apoptosis could not be promoted by composite material(P=0.988,P=0.919,P=0.781).Acute systemic toxicity test showed that the mice weight of experimental group [(33.30±3.81)g] had no statistical significance with control group [(31.08±2.91)g](P=0.166)and there was no death and toxicity effect in mice.Hemolysis test showed that the hemolysis rate of G/n-HA/PA66 composite was(0.97±0.36)%,which fitted the evaluation criteria of less than 5%(P=0.000).Pyrogen test showed that the increased temperature of experimental animal was(0.25±0.12)?,which fitted the evaluation criteria of less than 0.6 ?(P=0.035).Intradermal stimulation test showed that the phenomena such as erythema and edema were not observed around the injection point in the experimental group and control group,and the PII scores indicated the most slightly stimulation.Conclusion:G/n-HA/PA66 composite had excellent biocompatibility and practicability as a bone fixation implant.