Effect Of Expression Of Klotho On Regulation Of Mouse Osteoclast Differentiation

Objective To explore the effects of expression of klotho(KL)on the differentiation of RAW264.7 cells into osteoclasts induced by soluble receptor activator of nuclear factor-?B ligand(sRANKL).Methods The RAW264.7 cells were divided into Ad-KL group,Ad-GFP group and control group.The KL transfection into raw 264.7 cells was observed by inverted microscope.The expression levels of KL were detected by RT-PCR and Western blotting method.Tartrate-resistant acid phosphatase(TRAP)staining was applied to observe the osteoclast differentiation and maturation.The expression of TRAP and cathepsin K(CTSK)were assessed by RT-PCR and Western blot.Results Klotho gene was transfected into RAW264.7 cells successfully,Compared with Ad-GFP group and control group,the expression levels of KL mRNA and protein in Ad-KL group were increased(P < 0.01).The TRAP staining results showed that the number and volume of TRAP-positive cells in Ad-KL group was less than those in Ad-GFP group and control group.Compared with Ad-GFP group and control group,the expression levels of TRAP and CTSK mRNA(P <0.01)and proteins(P <0.05)in Ad-KL group were decreased.Conclusion The expression of klotho can inhibit the differentiation of osteoclasts.