Estrogen Recepter ESR1 Promotes BMSCS Cells Homing And Migration Via Regulation Of SDF-1/CXCR4 Signaling

Background:Stromal cell-derived factor-1(SDF-1)and its membrane receptor C-X-C chemokine receptor type 4(CXCR4)are involved in the homing and migration of multiple stem cell types which are essential for healthy endometrium.Herein,we investigated the mechanisms that overexpression of ESR1,which is a key factor in the pathogenic process of thin endometrium,could promote cell homing and migration of bone marrow-derived mesenchymal stem cells(BMSCs)via SDF-1/CXCR4 signaling.Methods: The lentivirus-transfected ESR1-overexpressed BMSCs were constructed for the mechanism study in the effects of ESR1 on the homing and migration of BMSCs.Result: We found that overexpression of ESR1 increased cell homing in BMSCs.In addition,it was also observed that ESR1 overexpression elevated expressions of SDF1,CXCR4.Activation of ESR1 further led to cell-cycle G1/S transition,which in turn directly upregulated cell cycle-related factors,such as cyclin D1.Taken together,the results indicated that activation of SDF1/CXCR4 signaling was involved in ESR1 induced cell homing and migration of BMSCs.Conclusion: These findings may better our understanding of the role ESR1 plays in the prevention of thin endometrium through affecting the homing and migration of BMSCs and promote the development of advanced treatments for series of diseases,including thin endometrium.