| Objective : The intraperitoneal injection of lipopolysaccharide(LPS)was used to construct a stable intrauterine model of intrauterine infection.The degree of injury induced by intrauterine infection was observed by lung biopsy.The levels of IL-6,IL-8,MMP9 concentration,to explore the value of these inflammatory factors in predicting lung disease.Methods:Intrauterine infection group: 18 pregnant rats 18 days,respectively,in the intrauterine injection of the appropriate concentration of LPS(0.6,0.7,0.8,0.9,1.0mg / kg).Control group: pregnant rats were pregnant with 3 days,intrauterine injection and infection group LPS equivalent sterilized saline.The number of surviving survivors of natural births was examined,each of which was weighed and weighed,and then fed with the mothers of the fetus to record the respiratory rate,the degree of motion and the presence or absence of cyanosis at different times of the day.After the delivery of placenta(natural delivery of placenta can be easily failed to choose,choose to choose fresh cesarean section to collect fresh placenta);according to 1,3,7 days of age were randomly selected in the two groups of natural births of the newborn rats Newborn rats were randomly divided into 48 lung tissue samples at each time point.The rats were sacrificed randomly.The rats were sacrificed and the blood samples were collected.Blood samples were taken for ELISA.The lungs were removed quickly and the placenta and lungs were taken for HE staining.Made sliced spare.Compared with the LPS group,the placenta and lung pathology scores of newborn rats were compared with those of the control group and the control group.The body weight / lung weight of the newborn rats was compared with that of the LPS group.The expression of IL-6,IL-8 and MMP-9 in fetal blood was not statistically significant.Result:1.The comparison of parameters of pulmonary function of tidal respiration:(1)When the concentration of LPS was more than 0.7mg / kg,the placental pathology of each group was significantly different from that of control group(P <0.05).Placental pathology showed that inflammatory cells began to infiltrate chorionic amniotic membrane,the higher the concentration,the more inflammatory cells(2)(P <0.05),suggesting that intrauterine infection can cause pulmonary inflammation,when the injection of LPS concentration(P <0.05),the difference was statistically significant(P <0.05),suggesting that intrauterine infection can cause pulmonary inflammation,when the injection of LPS concentration The higher the lung tissue infiltration of inflammatory cells,the more the destruction of lung tissue,the more severe lung injury in newborn rats,and this damage will continue to about 7 days after birth.(3)There was no significant difference in respiration rate and body weight / lung weight between the experimental group and the control group(P> 0.05).There was no significant difference between the experimental group and the control group(P> 0.05).(4)Compared with the control group,the levels of IL-6,IL-8 and MMP-9 in the blood of experimental group were statistically significant(P <0.05),and with the increase of LPS concentration,IL-6,IL-8,MMP-9 concentration is also getting higher and higher.Conclusions: SD rats injected intraperitoneal injection of LPS intraperitoneal injection of LPS could lead to intrauterine infection in pregnant rats.At the time of injection of LPS concentration of 0.7 mg / kg,stable intrauterine infection model could be made,and when LPS concentration was greater than or equal to 0.7 mg / Kg can cause neonatal lung pathological damage,pulmonary edema,and this damage will continue to 7 days after birth,blood levels of inflammatory factors IL-6,IL-8,MMP-9 will increase,Infection of lung disease has important value. |
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