The Research On The Effects And Mechanism Of Fractalkine On The Proliferation And Invasion Of Human Pancreatic Cancer Cell Lines By Regulating IL-6/AKT/Stat3 Pathways

Objective To explore the effects and mechanism of Fractalkine(FKN)on the proliferation and invasion of human pancreatic cancer cell lines PANC-1 and SW-1990 by regulating IL-6/AKT/Stat3 pathways.Methods Thetest included the control group,FKN-siRNA and FKN-siRNA negative group.Adenovirus mediated FKN-small interfering RNA(siRNA)was transfected into pancreatic cancer cell lines PANC-1 and SW-1990.The proliferation and invasion ability were detected by CCK-8 assay and Transwell assay.After FKN-siRNA transfection by pancreatic cancer cell PANC-1 and SW-1990,the expression of FKN,IL-6,AKT and Stat3 protein and mRNA level were detected by Western blot and RT-PCR.The data were analyzed by one way analysis of variance.Results(1)FKN was expressed in pancreatic cancer cell line PANC-1 and SW-1990,and FKN-siRNA was successfully transfected into pancreatic cancer cell line PANC-1 and SW-1990.(2)Through the CCK-8 method to detect cell proliferation activity,it showed that after transfected with FKN-siRNA for 12 hours and 24 hours,the absorbance values(OD)of each group in pancreatic cancer cell lines PANC-1 and SW-1990 had no significant changes.Transfected with FKN-siRNA for 48 hours and 72 hours,absorbance values in the FKN-siRNA group was significantly higher than that of the control group and FKN-siRNA negative group(P<0.05).(3)In the invasion ability assay,after pancreatic cancer cell lines SW-1990 and PANC-1 transfected with FKN—siRNA,it showed that the invasion ability of FKN-siRNA transfecting cells was stronger than that of control group and FKN-siRNA negative group(P<0.05).(4)The result of Western blot and RT-PCR showed that after FKN-siRNA transfection by pancreatic cancer cell lines PANC-1 and SW-1990,compared with the control group and the FKN-siRNA negative group,the expression ofFKN protein and mRNA in FKN-siRNA group decreased significantly(P<0.05).Meanwhile,the expression of IL-6,AKT and Stat3 protein and mRNA increased significantly in FKN-siRNA group(P<0.05).Conclusion 1.With siRNA technology to silent FKN function,the proliferation and invasion ability of human pancreatic cancer cell Lines increased,which indicates that FKN has a great effect on the biological activity of pancreatic cancer cells.2.Chemokine FKN affects the proliferation and invasion ability of pancreatic cancer cells by regulating IL-6/ Stat3 pathways.