The Role Of Sufentanil Preconditioning On Mapk Signal Pathway And Expression Of Connexin 43

Objective To evalute the role of Sufentanil preconditioning on MAPK signal pathway and expression of connexin 43Methods Primary cardiomyocytes were cultured for 5 days were randomly divided into 13 groups:control group(group C),hypoxia / reoxygenation(H / R group),morphine preconditioning group(group M),sufentanil preconditioning group(group S),ERK inhibitor group(group IE),JNK inhibitor group(group IJ),p38 MAPK inhibitor group(group IP),ERK inhibitor+morphine preconditioning group(group MEB),ERK,inhibitor +sufentani preconditioning group(group SEB),JNK inhibitor+morphine preconditioning group(group MJB),JNK inhibitor+sufentanil preconditioning group(group SJB),p38 MAPK inhibitor +morphine preconditioning group(group MPB),p38 MAPK inhibitor + sufentanil preconditioning group(group SPB).The cells were exposed to hypoxia solution saturated with mixture of 95% N2+5% CO2 for 2 h,and cultured in the normal atmosphere for 1h.Cx43 changes in each group were measured average optical density(AOD)by confocal microscopy;the Cx43 protein expression and Cx43-Ser368 sites phosphorylation calculated by Western Blot.Results: Compared with group C,the rest group of Cx43,p-Cx43Ser368 protein and AOD expression reduced(P(27)0.05);Compared with group H/R,Cx43,p-Cx43Ser368 protein and AOD expression were significantly increased in group M,group S,group MEB,group SEB,group MJB,group SJB,groupMPB,group SPB(P(27)0.05),group IE,group IJ,group IP showed no significant difference(P>0.05);compared with group M,Cx43,p-Cx43Ser368 protein and AOD expression decreased in group IE,group IJ,group IP,group MEB(P(27)0.05),group S,group MJB,group MPB were showed no significant difference(P>0.05);compared with group S,Cx43,p-Cx43Ser368 protein and AOD expression decreased in group IE,group IJ,group IP,group SEB(P(27)0.05),group SJB,group SPB were no significant difference(P> 0.05).Conclusion: Sufentanil pretreatment can produce similar protective effect on Cx43 with morphine,enhance the level of Cx43-Ser368 sites phosphorylation;This effect is associated with ERK signal pathway,also may be related to JNK,p38 MAPK signal pathway.