Protective Effect Of High Concentration Hydrogen Inhalation On Spinal Cord Injury In Mice

BACKGROUNDS: Spinal cord injury is one of the most serious complications of spinal injury,and it always lead to truncate the body under severe dysfunction.Pathogenesis of SCI involves many factors which can lead to tissue damage,but the immune inflammatory response plays a very important role during its pathogenesis.Although the optional means of prevention and cure of the disease are the following:surgical intervention,hormone shock treatment,drug therapy and cell transplantation therapy,et al.,the incidence of spinal cord injury in our country are still present the trend of rising year by year.Therefore,how to maximize the ease of spinal cord disability degree is still a hot issue of medical research.Since it was first reported by Ohsawa in 2007 that hydrogen could selectively neutralize cytotoxic oxygen species,over 300 studies have revealed the antioxidative features of hydrogen in different animal models.Local ischemia,inflammation and reactive oxygen species contribute to the secondary spinal cord injury and hydrogen could inhibit the secondary spinal cord injuries.This study summarized the current progress of hydrogen in the treatment of secondary spinal cord injuries.METHODS: Animal model of clamp-induced spinal cord injury was used in the present study.Male C57 mice were divided into 3 groups: Sham group,Spinal cord injury group and Hydrogen treatment group.The degree of spinal cord injury was evaluated by general observation,BMS score,footprint analysis,H&E and Nissl staining.RESULTS: Compared with Spinal cord injury group,the BMS score,footprint analysis and histological exam were significantly improved after hydrogen treatment..CONCLUSION: Inhalation of hydrogen has a protective effect against clamp-induced spinal cord injury in mice.Hydrogen breathing may be a potential strategy that can be applied in clinical practice.