Study On The Relationship Between Leptin And MMP-9 In Gastric Cancer Cells

Objective: The effect of leptin gastric cancer cells(SGC7901)of matrix metalloproteinase 9(MMP-9)correlation analysis the difference of mRNA gene transcription,which on leptin in gastric cancer cells and matrix metalloproteinase 9(MMP-9)correlation is expounded,and expect to provide a theoretical basis for the research of gastric cancer and clinical treatment.Methods: Human gastric cancer cell line SGC7901 using RPMI1640 culture medium containing 10% fetal bovine serum in 37 degrees Celsius,5%CO2 under the condition of conventional culture.The exponential students of human gastric cancer cells(SGC7901)by trypsin digestion after pounding after blending,one by one even join the petri dishes 24 hole,then genetic recombination anthropogenic sex after three steam distilled water dissolve uniform thin material added to a dish in the 24 hole,this experiment to gene recombinant anthropogenic leptin concentration respectively set up eight groups,respectively: control group,the 10 ng group,the 50 ng group,the 100 ng group,the 150 ng group,the 150 ng group,the 250 ng group,the 300 ng group.Each group has three repeat channel.Placed in 37 degrees Celsius,5%CO2 under the condition of regular training for 24 hours.After using I Real-Time polymerase chain reaction(PCR)in detection of human gastric cancer cells(SGC7901)sample leptin(leptin)under the influence of different concentrations of MMP-9 mRNA gene transcription level changes.Application of SPSS statistical software to analyze data obtained under different concentration groups matrix metalloproteinase 9(MMP-9)mRNA gene transcription level of median and the mean + /-standard deviation,according to the Real-Time PCR original test results,and use the computational formula which is 2-??ctto calculate relative quantitative formula,so each sample genes relative quantitative results can be calculated.Obtain difference levels of mRNA gene transcription,which are purpose of the each other's samples relative to the reference samples,and corresponding data analysis for the date by the Kruskal-Wallis H.Results: Using Kruskal-Wallis H statistical method to compare the above data,Test for P<0.05,the results are statistically significant,the results showed that the concentration of leptin(250ng,300ng)group and control group were different at the same time,the concentration of leptin(250ng,300ng)groups with the concentration of leptin(10ng)by different groups.According to the 2-??ctrelative quantitative calculation formula to calculate the original detection results of Real-Time PCR,and the gene of each sample relative quantitative results: PPARr gene relative expression in the control group: 1.333316,0.977019,0.767652;10 ng group PPARr gene expression quantity: 0.760776,1.194642,0.893853;50 ng group PPARr gene expression quantity: 1.180527,0.822674,0.689638;100 ng group PPARr gene expression quantity: 0.878753,0.75578,0.722084;150 ng group PPARr gene expression quantity: 0.706569,0.627592,0.743446;200 ng group PPARr gene expression quantity: 0.723143,0.515884,0.524015;250 ng group PPARr gene expression quantity: 0.603967,0.481726,0.338198;300 ng group PPARr gene expression quantity: 0.537623,0.287232,0.420464.By comparing the relative expression of PPARr gene,the relative expression of PPARr gene in the 250 ng group was significantly different from that of the control group and 10 ng group,and the relative expression of PPARr gene was statistically significant.And the relative expression of PPARr gene in 300 ng group is significantly different from that of control group and 10 ng group in PPARr gene.And draw the histogram of the above data,It can be seen that with the increase of leptin in each sample,matrix metalloproteinase 9(MMP-9)decreased.Through the statistical analysis of the relative expression of MMP-9 gene in the sample and the histogram,At the genetic level,the change of leptin concentration in human gastric cancer cells(SGC7901)was negatively correlated with the transcription level of mRNA gene of MMP-9.Conclusion:1.The level of mRNA transcription of the target gene leptin of matrix metalloproteinase 9(MMP-9)was regulated at the gene level,Thus,the protein expression level of the target gene of MMP-9 was affected,and then the invasion and metastasis of gastric cancer cells were indirectly affected.And indirectly affect the invasion and metastasis of gastric cancer cells.2.Leptin plays a negative role in the regulation of matrix metalloproteinase 9(MMP-9)in human gastric cancer.It was negatively correlated with MMP-9 in gastric carcinoma that hyper secretion of leptin had a certain inhibition effect on MMP-9.and because of its negative correlation with MMP-9 shows high secretion of leptin in gastric cancer inhibition of MMP-9.