Expression Of CIAPIN1 Gene And Its Correlation With Cyclin D1 In Bladder Cancer

Bladder cancer(BC)seriously threatens the life and health of the patients,which is one of the most common malignant tumors in the genitourinary system.Although the detection rate of bladder cancer in China is lower than Europe,with the improvement of people’s living standard,combined with the increasing of the life expectancy,medical level,and environmental pollution,the incidence of bladder cancer in China increased year by year.More than65% of patients are non-myometrial invasive bladder cancer(NMIBC)in the first diagnosis,and currently the main treatment is transurethral resection of bladder tumor(TUR-BT),but often leading to treatment failure because of the high recurrence rate.So it is important to diagnosis bladder cancer early to improve the survival rate of patients.The study of the molecular mechanism of bladder cancer can help us to find the pathogenesis of bladder cancer related genes from the molecular level,to explore the mechanism and sensitive and specific prognostic indicators,and then has important theoretical significance and clinical value to the diagnosis and treatment of bladder cancer.CIAPIN1(Cytokine-induced apoptosis inhibitor 1)is a newly identified cytokine-induced apoptosis inhibitor,and it has proposed as an attractive target for cell apoptosis resistance.CIAPIN1’s coding sequence contains 939 bp and encodes 312 amino acid residues with a molecular mass of 16.5 k Da.It is composed of a methyl transferase domain in the N-terminal region and a hypothetical Cx2Cx24Cx2 C Zn-ribbon-like motif in the C-terminal region.CIAPIN1 may not only suppress apoptosis induced by a variety of stimuli,including death receptor activation,growth factor withdrawal,ionizing radiation,viral infection and genotoxic damage,but also effectively regulate the tumorigenesis.Several studies have demonstrated strong CIAPIN1 expression in most human solid tumor types and hematological malignancies.Growing evidence suggests that high levels of CIAPIN1 expression in cancer cells are associated with clinicopathological variables of aggressiveness and may represent a prognostic marker for patient outcome.However,CIAPIN1 expression in bladder cancer and its role has not been reported.In this study,the expression of CIAPIN1 gene in bladder cancer and its correlation with survival rate were analyzed,which provided the theoretical basis for the diagnosis and treatment of bladder cancer.Objective: Investigate the expression of CIAPIN1 in bladder cancer and its correlation with survival rate.Methods:1 The total RNA was extracted from the tissues and the adjacent of the patients with bladder cancer radical surgery.2 Using q RT-PCR to detect the expression of CIAPIN1 and Cyclin D1 m RNA in bladder and adjacent tissues and the results were statistically analyzed.3 Pearson’s correlation analysis of q RT-PCR data was performed using Graphpad prism 5,and analyzed the linear correlation between CIAPIN1 and Cyclin D1.4 The expression of CIAPIN1 protein in bladder cancer and adjacent tissues was detected by Western blot and analyzed statistically.5 ATCG database(Onco Lnc)was used to investigate the relationship between the expression of CIAPIN1 and the survival rate of patients with bladder cancer,and to investigate the effect of CIAPIN1 on prognosis.Results:1 The transcription level of CIAPIN1 was significantly upregulated in bladder cancer tissues.The expression of CIAPIN1 in bladder cancer tissues and adjacent tissues was examined from the samples which collected from patients with radical urinary bladder resection in the Second Hospital of Hebei Medical University.The results of PCR showed that CIAPIN1 m RNA expression in bladder cancerwas generally higher than that in adjacent tissues(P <0.01).The results of q RT-PCR showed that Cyclin D1 m RNA expression in bladder cancer was generally higher than that in adjacent tissues.2 The transcriptional level of CIAPIN1 and Cyclin D1 was positively correlated in bladder cancer.The expression of CIAPIN1 and Cyclin D1 was detected by q RT-PCR,and statistical analysis was performed on Graphpad prism 5 by statistical Pearson’s correlation analysis.The results showed that CIAPIN1 and the expression of Cyclyin D1 m RNA in the bladder tissue were linearly correlated(R = 0.5153,P = 0.0168).3 The expression of CIAPIN1 protein was up-regulated in the development of bladder cancer.The total protein of cancer and adjacent tissues were extracted and detected by Western blot.The results showed that CIAPIN1 protein levels were significantly up-regulated in bladder cancer compared with adjacent tissues,and the expression of β-actin in cancer and adjacent tissues did not change significantly.Western blot showed that the expression of CIAPIN1 protein was significantly increased in bladder cancer tissues(P <0.05).4 The high expression of CIAPIN1 indicates a poor prognosis in bladder cancer.We explore the relationship between the expression of CIAPIN1 in bladder cancer and the survival rate of patients with Onco Lnc in TCGA database to search for their relationship.The results of the search from Onco Lnc showed that there was no significant difference in survival rate of patients with high or low expression of CIAPIN1 in bladder cancer within 5years.However,patients with low expression of CIAPIN1 had a 10-year survival rate of 50% whereas a high expression of CIAPIN1 was less than30%,with a significant difference(P = 0.0421).Conclusions:1 CIAPIN1 m RNA and protein levels in bladder cancer tissues were significantly higher than adjacent tissues,involved in the development ofbladder cancer.2 There was no significant difference between high expression and low expression of CIAPIN1 in survival rate within 5 years.But the 10-year survival rate of patients with high expression of CIAPIN1 was significantly lower than that of patients with low expression of CIAPIN1.