| Objective: By observing the Cigu Xiaozhi capsule regulation on nonalcoholic fatty liver disease rats PPAR ?,CAT gene expression,clarifying the treatment mechanism and target on lipid metabolism and oxidative stress perspective.Methods: Normally feeding 70 male Wistar rats(weighing 200 ± 20 grams)for a week then all rats were weighted and number by weight.Ten rats were selected randomly as control group and fed with normal diet;other rats were fed with high fat diet rats for ten weeks to establish the rat model.Ten rats were chosen randomly and sacrificed at end of the tenth week.Making Pathological section with the liver biopsy to check whether model replication is successful.After the success of modeling,according to the weight size,50 rats were randomly divided into model group,pioglitazone group,capsule group Cigu Xiaozhi(including high dose.Middle dose,low dose Group)5 groups,then fed with high-fat diet for 2 weeks.The whole modeling period was during for 12 weeks.After modeling pioglitazone group and the Cigu Xiaozhi Group were given by gavage with pioglitazone(pioglitazone group)or Cigu Xiaozhi(Cigu Xiaozhi Group)for 6 weeks,then the rats were sacrificed to collect liver tissue and serum,serum total cholesterol in serum triglyceride(TC),Triglycerides(TG),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C),alanine aminotransferase(ALT),aspartate aminotransferase(AST),free fatty acid(FFA)and peroxisome proliferator activated receptor changes in liver tissue(PPAR ?),catalase(CAT)gene expression.Results:(1)After 6 weeks of treatment,the weight increase of rats in model group was higher than that of rats in the blank group.There was significant difference(P<0.05)between the two groups;after the rats were sacrificed,statistical analysis was conducted on the liver weight of rats in the treatment group and liver weight of rats in the model group.The statistical results showed that the liver weight of the treatment group was significantly reduced and the difference was statistically significant(P<0.05).(2)Evaluating model group and liver steatosis in rats treated by HE staining and light microscopy,the difference between the two groups and was statistically significant(P<0.05).(3)Statistical compared with the blank group,The ALT,AST of model group rats increased significantly and the difference was significant(P<0.05);statistical analysis was conducted on ALT,AST of the treatment group and ALT,AST of model group,there was significant difference(P<0.05).(4)Serum TC,TG,LDL-C increased and HDL-C decreased in model group.Statistical analysis was performed with the control group and there are obvious differences(P<0.05);compared with the model group,drug treatment group rats TC,TG,LDL-C decreased and HDL-C increased,there were statistically significant(P<0.05).(5)Compared with the control group,serum FFA in model group increased significantly(P<0.05);after drug intervention,FFA of treatment group were significantly decreased,statistically compared with the model group,there were statistical significance(P<0.05).(6)The rat liver tissue PPAR ? and CAT gene expression of model group decreased statistically compared to the blank group,the statistical results significantly(P<0.05);After drug intervention groups,the expression of PPAR ? of liver tissue alpha and CAT gene in treatment group increased compared with the model rats,there was significant difference(P<0.05).Conclusion: 1.Cigu Xiaozhi capsule high dose group has a good effect of.2.Cigu Xiaozhi capsules each dose group increased by PPAR alpha NAFLD rats on weight loss of NAFLD rats,the expression of CAT gene can reduce the liver weight,blood lipid metabolism was improved,inhibit increase of liver enzymes,reduce lipid accumulation in the liver and reduce the content of FFA in serum. |
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