A Study On RSV Induced Immune Response Of COPD Patients

Objective: This study aims to investigate whether or not RSV can induce immune response of COPD patients through clinical trials.Methods: Firstly,the study collects sputum/blood samples of 30 subject from AECOPD group,SCOPD patients and normal people.Then,the sputum/Blood TLR3 mRNA and TLR4 mRNA are detected by fluorescence quantitative PCR,and three groups of subject with sputum RSV are detected by Nested PCR.and RSV positive group was detected RSV,negative group was not detected RSV.Secondly,the study collects clinical data from three groups of subject,including their age characteristics,blood gas,blood routine examination and lung function.The correlation analysis is used to detect the relativity among TLR3 mRNA and FEV1,and ELISA method is used to detect IL-13,IL-32,INF-β,TNF-α and IRF-3 protein expression.Results: 1.There were 8 RSV cases in AECOPD group,accounting for 26.6%,an d 3 RSV cases in SCOPD group,accounting for 10%.2.The inflammatory factors such as IL-13,IL-32,INF-β,TNF-α and IRF-3 pr otein expression level in RSV positive group were higher than those in RSV negati ve group(P< 0.05).3.The content of TLR3 mRNA and TLR4 mRNA in RSV positive group was higher than that in RSV negative group,which is statistically significant(P < 0.05).In RSV positive group,the expression of TLR3 mRNA in sputum was associate d with a negative correlation(r = 0.95)for the severity of COPD.Conclusion: The increases of TLR3 mNRA are closely related to reduction of pu lmonary function of AECOPD patients.TLR3 mRNA mediated antiviral effect can also produce inflammatory factor and induce immune injuries.The inflammatory fac tors in RSV group were significantly higher than those in the control group,suggest ing that RSV is an vital pathogenic cause of AECOPD,and RSV induced immune injury may be involved in the pathogenesis of COPD.