The Preparation And Properties Of Antimicrobial Alginate Hydrogel Base On Diels-alder Click Chemistry Reactions

The microbial infection,especially those associated with impaired wound healing and biomedical implant surgery,is one of the most common issue in clinical medicine.When the skin wounded,infection caused by microbial may prolong or impair the wound healing process leading to tissue necrosis.Regarding biomedical implants,infection at the implant–tissue interface can lead to implant failure.Three-dimensional network of hydrophilic hydrogel can absorb large amounts of water,can provide moist environment which can promote wound healing and its structural features very similar to the body’s soft tissue which is very suitable for implantable medical devices coating.We attempt to invite the hydrogel with antimicrobial properties for the clinical application of wound healing and implantable medical devices coating.In this paper,we have we have prepared alginate/PEG hydrogel by Diels-Alder click chemistry.By changing the mole ratio of alginate and furfurylamine,we can regulate the degree of substitution,control the crosslinking density of network and the swelling properties,topographic characteristic and mechanical properties.To improve the mechanical properties of the hydrogel,we incorporate ionic crosslinking with the Diels-Alder click chemistry by using Ca²⁺ to crosslink with the alginate to form double crosslinking hydrogel（DCgel）.The result show the mechanical properties of hydrogel have significantly improve.We can optimize the mechanical properties by changing the ratio of ionic cross-linking density to chenmical cross-linking density.In order to confer the antimicrobial properties to the hydrogel,this paper covalently modified the antimicrobial agent containing sulfhydryl group and the hydrogel by the thiol-ene reaction.Sulfhydryl group can react with the double bond of the product by Diels-Alder click chemistry under 365 nm UV light,I2959.TMC-CYS and HHC10-CYS covalently modified to the hydrogel which show strong sterilizing rate respectively 90% and100%.The hydrogel with the antimicrobial agent show better cell-adhesion ability.