| Objective: Metastasis is the most common cause of death in breast cancer patients.The most common metastatic sites are lung,bone and liver.Breast cancer angiogenesis is a key factor leading to the proliferation and metastasis of breast cancer.In this study,knee loading was used to observe the effect of knee loading on the lung metastasis of breast cancer in mice.Previous experiments have confirmed that knee loading is closely related to angiogenesis,inflammatory response and other closely related,but also with the bone metabolism and other related diseases also have a certain relationship,but the knee loading in the inhibition of lung cancer metastasis of breast cancer has not been reported.In this study,we established the model of breast metastasis of breast cancer,and observed the effect of knee loading on lung metastasis through the apoptosis of breast cancer cells and its effect on angiogenesis,and provided new ideas for the clinical treatment of lung metastasis of breast cancer.Research methods: 1.In vivo experiments were performed to amplify murine 4T1 cells with high metastatic rates to mimic human triple-negative breast cancer and to form a breast cancer model of breast cancer in the tail vein of female BALB/c mice weighing 18-22 g in 4-6 weeks.Were randomly divided into two groups,each group of 15.After 18 days of treatment,the mice were sacrificed consecutively and the left ventricular infusion group,Each slice thickness of about 5?m,ink infusion group,take the lung tissue for general observation.2.Organizational analysis: 1)HE staining analysis of tumor lung metastasis,analysis of mechanical loading and tumor apoptosis associated with.2)The number of tumor nodules was analyzed by ink perfusion,and the relationship between tumor metastasis and mechanical loading was analyzed.3)Immunohistochemical staining was used to detect the proliferation,apoptosis and angiogenesis of tumor cells in lung tissue.4)TUNEL method was used to detect the apoptosis of tumor,and the relationship between mechanical loading and tumor apoptosis was analyzed.5)To analyze the correlation between tumor cell apoptosis and tumor angiogenesis.6)All data were analyzed by SPSS statistical software.The quantitative data between the groups were compared with the t test,P<0.05.Each group of data to mean ± standard deviation(x ± s)to represent the application of office software and PS software for chart production.Research result: 1.Compared with the model group(21.24 ± 7.53%),the percentage of tumor area in the single field of view of the lung metastases(11.20 ± 4.05%)was significantly decreased(P<0.05).2.Compared with the model group,the number of lung metastases in the treatment group was significantly decreased(P<0.05).3.Ki67 results showed a significant decrease in Ki67 protein expression in the treatment group compared with the model group(P<0.05).4.CD31 results showed a significant reduction in the number of positive cells in the treatment group compared with the model group.5.TUNEL results showed that the number of TUNEL positive cells in the treatment group(39.25 ± 5.31)was significantly higher than that in the model group(57.25 ± 4.49)(P<0.01).6.The correlation results showed that tumor cell apoptosis and tumor angiogenesis have a certain correlation.Conclusion: In vivo experimental study shows that the model of tail vein pulmonary metastasis of 4T1 inoculated BALB / c mice is successful,and this model can provide valuable experimental tools for the study of lung metastasis of breast cancer.Through the experiment we can confirm that mechanical loading can inhibit the angiogenesis and increase the apoptosis of tumor cells in a certain extent.Through correlation analysis,it is found that tumor angiogenesis and apoptosis have a certain correlation,which is a clinical treatment of breast cancer lung metastasis provide new ideas. |
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