The Experimental Research Of Immunoregulation Effect On Tanshinone ?a In NMO Mice Animal Model

Background:Neuromyelitis optica spectrum disorder(NMOSD)is an autoimmune inflammatory disease of the CNS primarily affecting spinal cord and optic nerves.The clinical manifestations of NMOSDs are more serious,and the patients have high risk of disability which can threaten the patients' life.Therefor,the treatment of this disease has always been studied by clinicians and researchers.Tanshinone ?a has been broadly proved to have a lot of pharmaceutical activities in recent years.It can be used to cure a variety of autoimmune disease in central nervous system extensively,and it has a good command of immunoregulation,.Objective:In this experiment,we established the NMO animal model mimicing the pathogenesis of NMOSD,then treated with tanshinone ?a.We aim to explore whether tanshinone ?a can treat NMOSD.Methods:We choose the C57/BL6 female mice as the experimental animal.Dividing the mice into two groups,one of group is the experimental group(TSA group),the other one is the control group(Vehicle group).The experimental group is intervened by tanshinone ?a and the control group is intervened by PBS with0.4%DMSO.After 3 days,we established the NMO animal model.After 24 h we killed all the mice.then processed the brains to freezing sections.We analysed and compared the leisions with immunofluorence(AQP4/GFAP,MBP,CD45,Ly6 G,F4/80,TUNEL/Ly6G)..Result:Comparing to Vehicle group,the missing area of AQP4?GFAP?MBP in the lesion side hemisphere decreased significantly in TSA group,and the infiltration of CD45 +(total white blood cells),Ly / 6 g +(neutrophil),F4/80 +(microglial cells and macrophages)area is also reduced accordingly.The TSA group of neutrophil apoptosis ratio is significantly higher than control group(p < 0.05)Conclusion:TSA has remarkable immunoregulation effect on intervening the acute phase of NMO mouse model.It can inhibit the progression of lesions.TSA inhibits the loss of AQP4?GFAP?MBP by the way of inhibiting the inflammatory cell infiltrate into the CNS and promoting the apoptosis of neutrophil.TSA is expected to cure the autoimmune disease of CNS in clinical.