| Objective :This study explores the therapeutic effects that hyaluronic acid combined with minocycline hydrochloride in rats wound infected with staphylococcus aureus.Methods:1.The preparation of bacteria liquid: The S.Aureus ATCC 6538 strain were revived and the bacteria liquid of 1 x 108 cells/ml was prepared.2.Models were made and divided into groups: The full-thickness dermal excision model beside spine were established of 32 female wistar rats,wounds were inoculated with staphylococcus aureus to form infected wound.There were 4 groups of rat,8 rats and 16 infected wounds in each group,divided randomly.The experimental group(1.5 % hyaluronic acid combined with 0.1 % minocycline hydrochloride gel treatment group,n=8),the first control group(0.9 % normal saline treatment group,n=8),the second control group(1.5 % hyaluronic acid gel treatment group,n=8)and the third control group(0.1 % minocycline hydrochloride gel treatment group,n=8).3.Observations: The wound healing condition and the rate of that related to rats were watched and detected,through the method of HE staining to fulfill histological analysis and imunohistochemical staining of TNF-? were accomplished.4.Statistics analysis: With SPSS19.0 software to measure and it is meaningful(P < 0.05).Results:1.The rate of wound healing in the hyaluronic acid combined with minocycline hydrochloride group was(78.13?3.04)% significantly higher related to that of normal saline group(P<0.05),hyaluronic acid group(P<0.05)and minocycline hydrochloride group(P<0.05).2.There existed a great quantity of mature fibrous tissue in the hyaluronic acid combined with minocycline hydrochloride group;the tissue necrosis and unclear structure were observed in the normal saline group;in the hyaluronic acid group,the granulation tissue was rich in blood vessels;there existed a mass of fibrous tissue in the minocycline hydrochloride group.3.The expression of TNF-? in the hyaluronic acid combined with minocycline hydrochloride group was 10.84?1.49 significantly lower related to that of normal saline group(P<0.05),hyaluronic acid group(P<0.05)and minocycline hydrochloride group(P<0.05).Conclusions: Our study show that 1.5 % hyaluronic acid combined with 0.1 % minocycline hydrochloride gel can have a promising therapeutic effect on the rats wound infected with staphylococcus aureus. |
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