| Objectives To detect the expression of protein kinase B(p-AKT),S100 calcium-binding protein A4(S100A4)expression and the mutations of epidermal growth factor receptor(EGFR)gene in Non-small Cell Lung Cancer(NSCLC).Clinical information of these patients including age,sex,pathological type,smoking status,lymphnode metastasis,P-TNM stage and survival time was got by searching the medical records.To investigate the relationship and clinical significance of p-AKT,S100A4 expression and EGFR mutation.Methods 106 cases of NSCLC surgery specimens were collected from January 2011 to December 2015.And 25 cases of normal lung tissues were collected.Immunohistochemistry(SP)method Was used to detect the expression of p-AKT,S100A4.Before surgery,none of these patients was treated by chemotherapy or radiotherapy.EGFR mutation in exons 19 and 21 was detected by using the method of amplification refractory mutation system(ARMS).Clinical information and survival situation after surgery of these patients were maked a contrastive study.Statistical analysis was performed by using SPSS 21.0 statistical software package.Result 1.For the 106 NSCLC patient specimens,the positive expression of p-AKT and S100A4 was higer than that in normal tissue sample.2.The expression of p-AKT was associated with pathological type and P-TNM stage(P<0.05),The expression of S100A4 was associated with pathological type,lymph node metastasis and p-TNM stage(P<0.05).The mutation of EGFR was associated with sex,pathological type and smoking status(P<0.05).3.The expression of p-AKT have positive correlation with the mutation of EGFR(r=0.433,P<0.05),the expression of S100A4 have positive correlation with the mutation of EGFR(r=0.296,P<0.05)the expression of S100A4 have clear positive correlation with the expression of p-AKT(r=0.475,P<0.001).4.The expression of p-AKT can predict EGFR mutation in NSCLC,and the positive predictive value,the negative predictive value,the sensitivity,the specificity were 58.46%,85.37%,86.36%,56.45% respectively.The expression of S100A4 can predict EGFR mutation in NSCLC,and the positive predictive value,the negative predictive value,the sensitivity,the specificity were 52.17%,78.38%,81.82%,46.77% respectively.5.Univariate analysis in Cox model showed that P-TNM stage,lymph node metastasis,expression of p-AKT and S100A4 made difference to postoperative overall survival(P<0.05).Multivariate Cox model analysis in Cox model revealed P-TNM stage and expression of S100A4 were independent prognostic factors of NSCLC(P<0.05).Conclusion 1.The high expression of p-AKT and S100A4 are prevalent in the tissue of NSCLC,.The expression of p-AKT and S100A4 is correlated with the development of NSCLC.2.The positive expression of p-AKT、S100A4 and the rate of EGFR mutations are higer in female and adenocarcinoma patients 3.The expression of p-AKT and S100A4 and the mutations of EGFR are closely relayed in NSCLC,both of which have clear positive correlation.4.The negative expression of p-AKT、S100A4 can predict EGFR non-mutation in NSCLC in clinic.5.The double positive expression of p-AKT、S100A4 relates the worse prognosis of NSCLC.The expression of S100A4 in NSCLC is the independent predictive factor of prognosis. |
