Correlation Between The Expression Of CyclinD1 And P53 Protein In Different Histological Types And Organization Atypia Of Short Segment Barrett Esophagus

Objectives: To investigate the expression of CyclinD1 and P53 protein in different pathological classification,endoscopic morphology and organization atypia of short segment Barrett esophagus(SSBE),predict the risk of canceration in patients of SSBE,screen the patients with high-risk accurately,and provide an evidence for making endoscopic follow-up plan.Methods: Fifty-one cases with short segment Barrett esophagus(SSBE)were diagnosed by endoscopy,biopsy and pathological examination.Another 32 cases with normal esophageal mucosa were chosen as the control group.According to endoscopic morphology,the cases with SSBE were sub-grouped included circumferential type(16 cases),tongued-like type(13 cases)and island type(22 cases).According to histopathology,the cases with SSBE were classified to gastric fundus type(20 cases),cardia type(21 cases)and special intestinal metaplasia type(10 cases).According to organization atypia,the cases with SSBE were included non-dysplasia(37cases),low-grade dysplasia(14cases)and high-grade dysplasia(0cases).The expression of CyclinD1 and P53 protein were examined with the SP immunohistochemistry method and the results were statistically analyzed.Results: The expression of CyclinD1 and P53 protein in group of SSBE(CyclinD1 33.3%,P53 39.2%)and normal esophageal mucosa(CyclinD1 15.6%,P53 25.0%)has no statistically significant difference(p > 0.05).No remarkable differences about expression of CyclinD1 and P53 protein among the subgroups according to endoscopic morphology and histopathology were found(p>0.05).The expression of CyclinD1 and P53 protein was all in high level in group of SSBE with dysplasia.The positive rates of CyclinD1 in non-dysplasia group,low-grade dysplasia group were 21.6%,64.3% respectively,the difference between groups was statistically significant(p=0.007).The positive rates of P53 protein in non-dysplasia group,low-grade dysplasia group were 27.0%,71.4% respectively,there was significant difference between the two groups(p=0.006).Conclusions: SSBE has low risk of canceration.The risk of cancer in SSBE has no direct correlation with morphologic classification under endoscope and histopathological classification.But with the increase of dysplasia degree,the expression of CyclinD1 and P53 protein increased obviously,which prompts that cancerization risk in SSBE with dysplasia will increase significantly.