The Role Of C-IAP1 And P-gp In Predicting The Efficacy Of Neoadjuvant Chemotherapy For Locally Advanced Cervical Cancer

Objective:Testing the relative content variation of c-IAP1 and P-gp mRNA and protein expression in different treatment groups in cervical squamous cell carcinoma by Western blot and RT-PCR before and after NACT,and then explore the relationship between the expression level of the c-IAP1 and P-gp and the effect of chemotherapy,and speculate whether the two can be used as a biological indicator to predict the efficacy of NACT in patients with cervical cancer.Method:From 2015.03 to 2016.07 the 30 cases locally advanced cervix squamous cell carcinoma patients was recruited from gynecology and obstetrics clinic of the second hospital of Shanxi Medical University the second hospital of Shanxi Medical University,which were diagnosed by colposcopy and cervical biopsy,the samples after taken were immediately frozen in liquid nitrogen.Pathologically confirmed or clinically suspected,combined with other malignancies,hysterectomy,and prior to receiving hormone,chemoradiotherapy,etc,these were excluded from this study.There were no contraindications to chemotherapy,communicate fully with the patient and obtain consent,2 courses of TC regimen(paclitaxel + carboplatin)chemotherapy were performed,the short-term effects of chemotherapy were observed continuously,After operation,the tissue was taken from the lesion of cervix cancer and frozen into liquid nitrogen,application of Western blot into liquid nitrogen.Testing the relative content variation of c-IAP1 and P-gp mRNA and protein expression in different treatment groups in cervical squamous cell carcinoma by Western blot and RT-PCR before and after NACT.The data obtained by statistical software SPSS 19 related statistics and analysis.Result:1.The clinical effective rate of NACT was 83.3%(25/30).2.The relative expression of c-IAP1 of mRNA in effective group of locally advanced cervical squamous cell carcinoma before and after NACT were 1.085±0.418,1.459±0.595,invalid group were 1.859±0.535,2.427±0.370,c-IAP1 protein before and after chemotherapy in effective group were 0.063 ±0.039,0.237 ±0.208,invalid group were 0.143±0.027,0.571±0.092;P-gp mRNA in effective group before and after NACT were 1.281±0.882,2.021±0.883,invalid group were 2.814±0.706,4.807±0.656,P-gp protein before and after in effective group NACT were 0.899±0.075,2.340±0.613,invalid group were 1.324±0.192,3.285±0.844,the expression level of c-IAP1 and P-gp in cervical squamous cell carcinoma tissues after neoadjuvant chemotherapy were higher than that before chemotherapy,and the differences were statistically significant(P = <0.05).3.Before NACT effective group and ineffective group of cervical squamous cell carcinoma c-IAP1 mRNA expression levels were 1.085±0.418,1.859±0.535,c-IAP1 protein were 0.063±0.039,0.143±0.027;P-gp mRNA were 1.281±0.882,2.814±0.706,P-gp protein were 0.899±0.075,1.324±0.192,there were significant difference in the expression level of c-IAP1 and P-gp in the clinical effective group and ineffective group(P = <0.05),and the expression level of them in the effective group were lower than that in the ineffective group.4.There was no correlation between the expression of c-IAP1 and P-gp before NACT(P>0.05),Conclusion:1.The expressions of c-IAP1 and P-gp in the cervical squamous cell carcinoma tissues were significantly different before and after chemotherapy,and the two may be the potential biological indicators for the evaluation of the efficacy of NACT.2.The high expression of c-IAP1 and P-gp in locally advanced squamous cell carcinoma of the cervix may indicate that the patient is not sensitive to NACT,and may be helpful for clinical predictive chemotherapy.