The Mechannism Of Ultra-early Massage In Acute Skeletal Muscle Injury

Objective: To observe the effect of ultra-early massage on the expressions of membrane repair associated protein dysferlin in acute skeletal muscle injury rats,and demonstrate a possible mechanism how ultra-early massage protect myocytes from skeletal muscle contusion.Method: Fifty-five SD rats were randomly divided into normal control group(n = 5),natural recovery group(n = 25)and massage group(n = 25).Rats in the natural recovery groups and the massage groups were sacrificed on 1 d,2 d,3 d,5 d,7 d of treatment.There are 5 rats in subgroups at each time point.Muscle tissue was harvested in the region of the marked muscle.Morphological changes in the skeletal muscle tissues were observed using HE stain.The expression level of dysferlin was detected by Western Blot.Result: HE: Compared with the normal control group,muscle fiber blunt swelling appeared at 1 d and 2 d after skeletal muscle contusion.Inflammatory cells of the natural recovery group was more than that of the massage group in 3 d.More inflammatory cells were observed in 5 d.Inflammatory cells in 7 d massage group significantly decreased compared with that in 5 d.Less extensive fibrosis was observed in massage group than natural recovery group in 7 d.WB : The expression level of dysferlin in the model groups was significantly higher than that in the normal control group.The expression of dysferlin in the massage group was significantly higher than that in the natural recovery group at each corresponding time(p<0.5).The level of dysferlin in the subgroups of the massage group and the natural recovery groups gradually increased with the time from 1 d to 7 d.Conclusion: In course of acute contusion,ultra-early massage can increase expressions of dysferlin,promote the timely repair of ruptured membrane,decrease muscle injury induced and promote the repair of damaged skeletal muscle.