7,8-Dihydroxyflavone Ameliorates Motor Deficits VIA Suppressing ?-Synuclein Expression And Oxidative Stress In The MPTP-Induced Mouse Model Of Parkinson's Disease

Object: To determine that DHF exist protective effects on MPTP induced the loss of dopaminergic neurons,deficit of motor functions,the expression of ?-synuclein and antioxidative activity in the C57BL/6 mice.Methods:Administrated with MPTP(30 mg/kg,i.p.)5 days to induce the death of dopaminergic neuron in the SN in mice(20-25g).Meanwhile,DHF(5 mg/kg,i.p.)was treated daily for 13 days until the laster behavioral tests.Subsequently,rotarod test,pole test and wire suspension test were used to examine the effect of the DHF on PD behaviors.After behavioral tests mice were sacrificed,the tissures were collected to do immunoblot and immunohistochemical assays.Furthermore,anti-oxidative activity was also examined in four groups.Results: Behavioral tests showed that DHF significantly ameliorated the deficit of motor functions in the MPTP treated mice.The immunohistochemical date showed that DHF obviously reduced the loss of dopaminergic neurons in the SN and ST.Western blot analyses revealed that DHF suppressed the overexpression of ?-synuclein and prevented TrkB inactivation.Anti-oxidative activity detection showed that DHF prevented the decrease of glutathione and the reduction of total superoxide dismutase activity induced by MPTP.Conclusion: Therefore,the present results suggest that treatment with DHF may suppress the overexpress of ?-synuclein and oxidative stress via activating TrkB and blocking the loss of dopaminergic neurons in the SN and ST,subsequently ameliorating motor deficits induced by in the C57BL/6 mice.