Fasudil Protection On Myocardial Injury In Experimental Study Of Adrismycin

Objective:To study the killing effect of adriamycin combined with fasudil on K562 cells during chemotherapy in leukemia patients and the protective effect on cardiomyocytes.Method: K562 cells and HCM cells were selected for logarithmic growth,respectively for drug intervention,set up a control group A1 and A2,use adriamycin group B1 and B2,adriamycin combined with fasudil group C1 and C2,adriamycin joint dexrazoxane group D1 and D2(positive control group),after the intervention of cells under inverted microscope respectively by cells from dyeing experiment testing form,determined by MTT colorimetry to detect cell activity,current detection,apoptosis,western for testing the Bcl-2 cells inhibit apoptosis and promote the expression of apoptosis gene Bax.Result:The results of the K562 cell experiment:Inverted microscope A1 compared with control group,experimental group B1,C1,D1 cells from dyeing experiment are all positive;determined by MTT colorimetric method measuring cell activity in A1 compared with control group,experimental group B1,C1,D1 cell activity are reduced,and the absorbance scatter plot can be seen in three groups of experimental group K562 cells absorbance is similar;Flow detection,apoptosis rate and the control group in A1((X = 4.93%)compared to the experimental group B1((X = 81.9%),C1((X = 78.3%),D1((X = 86.0%)apoptosis rate increased significantly,and the difference between the three groups is not significant difference;Western bands graphic control group A1 and group B1,C1,D1 internal stripe ?-Actin basic equal strength,apoptosis suppression gene Bcl-2 banding strength in the control group A1 strength is higher,in the three experimental groups B1,C1,D1 band intensity was lower than those of A1 group,the banding of the proapoptotic gene Bax was lower in the control group A1,the strength of B1,C1 and D1 in the three groups were higher than those in the A1 group.The experimental results for HCM cells:Compared with control group A2,the experimental group B2,C2,D2 cells were positive for the rejection test,and the morphology of cells in C2 and D2 group was less than that in B2 group;MTT colorimetric assay showed that the activity of B2,C2 and D2 cells in experimental group was lower than that in control group A2,and the absorbance of HCM cells in C2 and D2 group was similar and higher than to that in group B2;flow detection,apoptosis rate and the control group in A1((X = 0.13%)compared to the experimental group B1((X = 42.5%),C1((X = 24.1%),D1((X = 23.7%) apoptosis rate increased significantly,and C2 and D2 between apoptosis rate and lower than similar B2 group;Western belt A2 graphic control group and experimental group B2,C2 and D2 internal stripe beta Actin basic equal intensity,suppression of apoptosis gene Bcl-2 band intensity in the control group A1 intensity is the highest,followed by C2 and D2,in group B2 intensity is the lowest,,the banding of the proapoptotic gene Bax was the lowest in the control group A2,The intensity of B2,C2 and D2 in the experimental group was higher than that in A2,C2 and D2 bands were higher,and the band strength of group B2 was the highest.Conclusion:in chemotherapy of leukemia,adriamycin and fasudil without obvious drug antagonism,combined with no reduction in the effect of chemotherapy on the basis of myocardial cells have a protective effect.