| Objective: To investigate the expression of the inhibitor kappa B kinase alpha(IKKα)and androgen receptor(AR)in human prostate cancer(PCa)and benign prostatic hyperplasia(BPH),provide a theoretical basis for further explore its role in the development and progression of prostate cancer.Methods:1 The IKKα and AR expression in 30 cases of BPH tissues and 30 cases of PCa tissues were detected by immunohistochemistry SP metheod.Analyze the expression of IKKα and AR in different tissues.To analysis the correlation between the IKKα and AR expression and their relationship with the grading,staging of PCa.2 Statistical analysis: The chi-square test and rank correlation analysis of SPSS 17.0 software were used for statistcal analysis of the results.The probability(P)-value less than 0.05 were cansidered to be statistically significant.Result:1 The immunohistochemical technique observation: Immunohistochemical results show that IKKα mainly localize in cytoplasm of glandular epithelium;AR mainly localize in the nucleus of glandular epithelium.The positive expression rate of IKKα in PCa group is 73.33%,which is significantly higher than that in BPH group(46.67%),the difference is statistically significant(P <0.05).The positive expression rate of AR in PCa group is 83.33%,which is significantly higher than that in BPH group(56.67%),the difference is statistically significant(P <0.05).2 The correlated statistics of IKKα and AR expression: the correlation coefficient of IKKα and AR expression is r=-0.568 in PCa group,has statistically significant(P <0.05),indicated a moderate degree of negativestatistical correlation between IKKα and AR expression.3 The correlated statistics of expression IKKα with tumor grading,staging of PCa group: The positive expression rate of IKKα in the poorly differentiated group is 93.33%,which is significantly higher than that in the well differentiated group(53.33%),the difference is statistically significant(P <0.05).The positive expression rate of IKKα in the advanced group is93.75%,which is significantly higher than that in the early stage of PCa group(50.00%),the difference is statistically significant(P <0.05).4 The correlated statistics of expression AR with tumor grading,staging of PCa group: The positive expression rate of AR in the well differentiated group is 100%,which is significantly higher than that in the poorly differentiated group(66.67%),the difference is statistically significant(P<0.05).The positive rate of AR in the early stage of PCa group is 92.86%,and the positive expression rate of AR in advanced group is 75.00%,the difference is not statistically significant(P> 0.05).Conclusion:1 The positive expression rate and intensity of IKKα and AR in prostate cancer tissues was significantly higher than that in benign prostatic hyperplasia tissues,indicating that IKKα and AR were closely related to prostate cancer.Detection of IKKα,AR is helpful in the early diagnosis of prostate cancer.2 With the increase of PCa malignant degree the expression of IKKαtend to increase,while the expression of AR tend to reduce.IKKα and AR could be used as an index to judge the prognosis of prostate cancer.3 The expression of IKKα and AR is closely related to the biological behavior,pathology of prostate cancer.Further study of its mechanism may provide new ideas or methods for the treatment of prostate cancer. |
