Effect Of Modified Qigesan On Epithelial Mesenchymal Transition Of Esophageal Cancer Cell TE1

In China,the incidence of esophageal cancer and mortality accounted for more than half of the global total incidence,so China focus on esophageal cancer prevention and treatment.In Hebei,the incidence of esophageal cancer is high,such as Shexian,Cixian,Wu An.According to the relevant departments of statistics,the incidence of esophageal cancer in Hebei Province is the incidence of esophageal cancer more than 4 times.Hebei Provincial Tumor Hospital as the largest tumor hospital in Hebei Province,the primary task of scientific research is the prevention and treatment of esophageal cancer.At present,surgery is the treatment of esophageal cancer commonly used method,only 5-year survival rate of surgical treatment is less than 20.0%,and the recurrence rate within 22% after surgery.We believe that the core pathogenesis of esophageal cancer is the stomach and blood depletion,so qi-stagnation,blood stasis,phlegm are appearance.We propose that the treatment of esophageal cancer is to increase the stomach yin.In the treatment of esophageal cancer patients,we use modified Qigesan delayed cancer recurrence time and inhibit the development of advanced cancer patients.This study explores the effect of modified Qigesan on the Epithelial mesenchymal transition of esophageal epithelial cells.Reveal the mechanism of inhibiting the invasion and metastasis of esophageal cancer cell.Objective: In Epithelial mesenchymal transition,modified Qigesan affect esophageal cancer cells.The results show that modified Qigesan restrain epithelial mesenchymal transition of esophageal cancer cells.It can inhibit the migration and invasion of esophageal cancer cells,reduce the occurrence of esophageal cancer metastasis.Methods:1 In this study,we selected highly differentiated esophageal cancer cells TE1,and divided into three groups:Control group,modified Qigesan50?g/ml,modified Qigesan 100?g/ml.Cells after 24 h of drug administration,western blotting was used to detect the expression of snail protein,cytokeratins14 and vimentin in three groups of esophageal cancer cell.2 After 24 h of drug administration,we used a laser confocal microscope to observe the cell structure in Control group,modified Qigesan 50?g/ml and modified Qigesan 100?g/ml.3 After 24 h of drug administration,we used Scratch test to observe the migration of TE1 in Control group,modified Qigesan 50?g/ml and modified Qigesan 100?g/ml.4 After 24 h of drug administration,we used Transwell to observe the invasion of TE1 in Control group,modified Qigesan 50?g/ml and modified Qigesan 100?g/ml.Results:1 Western blotting was used to detect the expression of snail protein,cytokeratins14 and vimentin in TE1.The results of snail protein showed that the control group was 0.150 ± 0.070,the modified Qigesan 50?g/ml was 0.121± 0.003,and modified Qigesan 100?g/ml was 0.034 ± 0.004.The results of vimentin protein showed that the control group was 0.163±0.005,the modified Qigesan 50?g/ml was 0.128±0.005,and modified Qigesan 100?g/ml was0.055±0.008.The results of cytokeratins14 protein showed that the control group was 0.035±0.003,the modified Qigesan 50?g/ml was 0.038±0.003,and modified Qigesan 100?g/ml was 0.033±0.001.2 Laser confocal microscopy was used to detect the effect of modified Qigesan on the cell structure of TE1.Control group of cell morphology was spindle-shaped or spindle-shaped irregular interstitial cell morphology.When the concentration of modified Qigesan 50?g/ml,than the control group more liked a circular or square cell morphology.When modified Qigesan 100?g/ml,TE1 was an epithelial cell morphology of round or square.3 Scratch test was used to detect the effect of modified Qigesan on TE1 migration.After 12 h of drug administration,the results showed that the control group was 35.366±1.665,the modified Qigesan 50?g/ml was 57.166±2.000,and modified Qigesan 100?g/ml was 77.566±3.066.After 24 h of drug administration,the results showed that the control group was 26.633±2.753,the modified Qigesan 50?g/ml was 35.133±3.780,and modified Qigesan100?g/ml was 46.366±2.375.4 Transwell was used to detect the effect of modified Qigesan on TE1 invasion.After 24 h of drug administration,the results showed that the control group was 105.626±3.785,the modified Qigesan 50?g/ml was 65.523±5.131,and modified Qigesan 100?g/ml was 25.366±4.131.Conclusions:1 The expression of snail protein and vimentin proteinwas inhibited by modified Qigesan(P<0.05).When modified Qigesan 100?g/ml,the inhibitory effect of snail protein and vimentin protein was better than that of modified Qigesan 50?g/m.2 The expression of cytokeratins 14 did not increase the expression by modified Qigesan(P> 0.05)3 After drug administration,esophageal cancer cells from spindle-shaped or spindle-like interstitial cell morphology into oval or square epithelial cell morphology.4 After drug administration,migration of esophageal cancer cells was inhibited by scratch test(P<0.05).When modified Qigesan 100?g/ml,the inhibitory effect of migration of esophageal cancer cells was better than that of modified Qigesan 50?g/ml.5 After drug administration,invasion of esophageal cancer cells was inhibited by scratch test(P<0.05).When modified Qigesan 100?g/ml,the inhibitory effect of invasion of esophageal cancer cells was better than that of modified Qigesan 50?g/ml.