The Functional Connectivity Study Of Resting-state MRI In The Comparation Of The Young People And The Ederly

Objective:To investigate the differences in the pattern of functional connectivity(FC)of cerebellum subregions between the elderly and young healty subjects using resting-state functional magnetic resonace imaging,and to reveal the impact of aging on the functional connectivity(FC)of cerebellum subregions;provide the evidence that the cerebellum have the nonmotor function indirectly and to undersd and the effect of normal brain aging on cerebellar function.Methods: 121 normal right-hands subjects were divided into the following two groups: the young group including 60 participantes(23 males,37 females,range from 22 to 3l years,mean age:26.2±1.9 years);the elderly group including 61 participantes(23males,38 females,range from 45 to 65 years,mean age:52.6±5.9 years).GE Signa Excite 3.0T superconducting MR Scanner was used to obtain resting-state f MRI data.DPARSFA,SPM8 and REST1.8 software,which were based on the Matlab platform,were used to preprocess all the date we acquisitioned,FC computation and statistical analysis.Preprocessing includes Slice Timing,Realign,Normalize,Smooth,Detrend,Covariates Removed and Filter.In the AAL template,the cerebellum consists of 26 subregions,which were used as the region of interest(ROI)to analyze the functional connectivity respectively,and then get the functional connectivity map.The statistical analysis was performed with two sample t-test,and then we observed the differences of functional connectivity between the elderly and the young in resting-state(P < 0.01),after alphasim correction(rmm=5).FC maps were overlapped onto the Ch2 bet standard structrual template to observe the locationg of the clusters,the peak MNI coordinate and the peak intesnsity.Results:1 Compared with the young people,the functional connectivity decreased brain regions between the cerebellum and cerebrum in the eldly including:1.1 In the cerebellar vermis region 1 /2(ROI1),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral precentral gyrus,postcentral gyrus,cuneus,superior temporal gyrus,lingual gyrus,middle occipital gyrus,inferior occipital gyrus;the left superior occipital gyrus,fusiform gyrus,and the right insula.1.2 In the cerebellar vermis region 3(ROI2),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral middle occipital gyrus,the right inferior occipital gyrus and the left lingual gyrus.1.3 In the cerebellar vermis region 4/5(ROI3),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding: the bilateral postcentral gyrus,the right precentral gyrus and the left orbital frontal gyrus,medial frontal gyrus,dorsolateral frontal gyrus,the right medial frontal gyrus.1.4 In the cerebellar vermis region 6(ROI4),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding: the right dorsolateral frontal gyrus and the left inferior temporal gyrus,rectus gyrus,precuneus.1.5 In the cerebellar vermis region 7(ROI5),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral superior frontal gyrus,orbital middle frontal gyrus and the right postcentral gyrus.1.6 In the cerebellar vermis region 8(ROI6),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral postcentral gyrus,calcarine cortex,orbital middle frontal gyrus and the right opercular inferior frontal gyrus,inferior temporal gyrus,fusiform gyrus,the left inferior occipital gyrus.1.7 In the cerebellar vermis region 9(ROI7),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral precentral gyrus,postcentral gyrus,calcarine cortex;the left rectus gyrus,lingual gyrus,fusiform gyrus and the right middle temporal gyrus.1.8 In the cerebellar vermis region 10(ROI8),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral postcentral gyrus,the right precentral gyrus;the bilateral superior occipital gyrus,inferior occipital gyrus,orbital superior frontal gyrus,fusiform gyrus and the right inferior temporal gyrus,middle temporal gyrus.1.9 In the left cerebellar hemisphere region 3(ROI9),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral precentral gyrus,postcentral gyrus,superior occipital gyrus,middle occipital gyrus,inferior occipital gyrus,cuneus,fusiform gyrus,middle temporal gyrus;the right inferior temporal gyrus,middle frontal gyrus;the left superior frontal gyrus,middle frontal gyrus,lingual gyrus,inferior parietal gyrus.1.10 In the right cerebellar hemisphere region 3(ROI10),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral precentral gyrus,postcentral gyrus,lingual gyrus,cuneus,middle temporal gyrus,inferior temporal gyrus,fusiform gyrus,superior occipital gyrus,middle occipital gyrus,inferior occipital gyrus,insula;the left midbrain,right supplementary motor area and the right medial superior frontal gyrus.1.11 In the left cerebellar hemisphere region 4/5(ROI11),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the right precentral gyrus,postcentral gyrus,opercular inferior frontal gyrus;the right medial superior frontal gyrus,hippocampus;the left insula,superior temporal gyrus.1.12 In the right cerebellar hemisphere region 4/5(ROI12),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral angular gyrus,the left middle frontal gyrus and the right medial superior frontal gyrus,orbital inferior frontal gyrus.1.13 In the left cerebellar hemisphere region 6(ROI13),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the left superior temporal gyrus pole,the bilateral insula and the right hippocampus,superior frontal gyrus,thalamus,middle cingulate gyrus.1.14 In the right cerebellar hemisphere region 6(ROI14),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding: the bilateral postcentral gyrus,hippocampus;the left parahippocampal gyrus,cuneus,precentral gyrus,the right opercular inferior frontal gyrus,triangular inferior frontal gyrus and the right middle temporal gyrus.1.15 In the left cerebellar hemisphere region 7b(ROI15),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the corpus callosum,the bilateral anterior cingulate gyrus,fusiform gyrus,parahippocampal gyrus;the left rectus gyrus,orbital inferior frontal gyrus;the right medial frontal gyrus,orbital middle frontal gyrus.1.16 In the right cerebellar hemisphere region 7b(ROI16),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the left fusiform gyrus,superior temporal gyrus pole,middle temporal gyrus pole;the right medial frontal gyrus and corpus callosum.1.17 In the left cerebellar hemisphere region 8(ROI17),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the brainstem;the bilateral fusiform gyrus,calcarine cortex;the right insula and the left orbital superior frontal gyrus.1.18 In the right cerebellar hemisphere region 8(ROI18),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:brainstem,medulla oblongata,the corpus callosum;the left thalamus,putamen,rolandic operculum;the right insula,middle occipital gyrus.1.19 In the left cerebellar hemisphere region 9(ROI19),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the right parahippocampal gyrus and middle occipital gyrus.1.20 In the right cerebellar hemisphere region 9(ROI20),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral medial superior frontal gyrus,the right superior temporal gyrus,the calcarine gyrus.1.21 In the left cerebellar hemisphere region 10(ROI21),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the right postcentral gyrus,orbital superior frontal gyrus,Heschl gyrus,rolandic operculum;the left rectus gyrus and the bilateral insula.1.22 In the right cerebellar hemisphere region 10(ROI22),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the blateral postcentral gyrus,insula;the right precentral gyrus,fusiform gyrus,cuneus,superior occipital gyrus,middle occipital gyrus,inferior occipital gyrus,calcarine cortex;the left superior temporal gyrus,lingual gyrus,superior occipital gyrus and the left thalamus,putamen.1.23 In the left cerebellar hemisphere region crus1(ROI23),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the bilateral precentral gyrus,postcentral gyrus,superior temporal gyrus,middle cingulate cortex,supplementary motor area;the left side of the brainstem,the left thalamus,superior frontal gyrus,insula and the right middle frontal gyrus.1.24 In the right cerebellar hemisphere region crus1(ROI24),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding: the bilateral middle cingulate cortex,lentiform nucleus,putamen,postcentral gyrus;the right rolandic operculum,and the left thalamus,the left side of brainstem.1.25 In the left cerebellar hemisphere region crus2(ROI25),campared with the youny,the elderly showed weaker functional connectivity brain areas concluding:the right parahippocampal gyrus,the right fusiform gyrus,the left middle frontal gyrus,the brain stem,and the left thalamus.1.26 In the right cerebellar hemisphere region crus2(ROI26),campared with the youny,the elderly showed weaker functional connectivity brain areasconcluding:the left superior temporal gyrus,the right lingual gyrus and the right parahippocampal gyrus.2 Compared with the young people,the functional connectivity enhanced brain regions between the cerebellum and cerebrum in the elderly mainly distributed in the middle frontal gyrus,inferior frontal gyrus,inferior temporal gyrus,middle temporal gyrus,inferior parietal gyrus,supramarginal gyrus and angular gyrus.Conclusion:1 Compared with the young,in the elderly,the resting-state functional connectivity decreased brain regions between the cerebellum and the cerebrum were very widely,which invoved almost all resting-state networks,instead of only the sensorimotor network.2 Compared with the young people,in the elderly some of the functional connectivity weakened brain regions between the cerebellum and the cerebrum belonging to the circuit of cerebrum-cerebellum.3 The effect of age on the functional connectivity of the bilateral cerebellar hemisphere were asymmetry.4 Compared with the young people,there are some functional connectivity enhancd brian regions in the elderly,which may be the result of the functional compensation.