Observation Of In Vitro Antibacterial Activity Of Galla Chinensis Ect Against 130 Strains Of Clostridium Difficile

Objective: Clostridium difficile is a kind of gram positive anaerobic bacillus,which can be divided into two kinds: the producing strain and the non producing strain.The producing strain is more harmful than the non producing strain,both of them can be planted in the human intestinal tract.Clostridium difficile is the main pathogenic bacteria causing antibiotic associated diarrhea.The spores can be resistant to a variety of physical and chemical factors,including alcohol disinfectants,moist soil,air,etc.As a medium of communication,Clostridium difficile can cause epidemic transmission in the community and hospital,the incidence of hospital acquired and community acquired Clostridium difficile infection and the severity of the disease are on rise in the world.Since twenty-first Century,the outbreak of Clostridium difficile high yield strain 027/NAPl/BI in Canada,North America,Europe and other regions,the mortality and recurrence rate is high.The current guidelines recommend that patients with mild to moderate Clostridium difficile take oral metronidazole as the primary,while the severe patients with oral vancomycin.However,the two drugs are easy to relapse after treatment,and the side effects are relatively large.In 2011,the FDA approved feldamycin for the treatment of Clostridium difficile,feldamycin has a significant effect,but the price is very expensive,which limits its clinical application.China is a big country of traditional Chinese medicine,traditional Chinese medicine has a long history in China,many of which have anti-inflammatory and antibacterial effects.We tested the antibacterial activity of Galla chinensis,catechu,Coptis chinensis,Scutellaria baicalensis Georgi and Forsythia suspense in vitro.In order to provide ideas and methods for traditional Chinese medicine treatment of Clostridium difficile infection.Method:1 A total of 130 strains of Clostridium difficile were collected from HBCMCC.2 The genome of Clostridium difficile was extracted,Amplification of16S-23 Sr DNA gene spacer.in order to determine the PCR-ribotype of CD according to the specific position of the electrophoresis bands.The toxin gene was amplified by multiplex PCR,and the production of Clostridium difficile was determined by electrophoresis.We explore the relationship between MIC of traditional Chinese Medicine and PCR-ribotyping.3 Deterimination of antibacterial activity of Galla Chinensis against 130 strains Clostridium difficile was performed by using agar dilution method,In order to determine the minimum inhibitory concentration of Galla chinensis,rhizoma coptidis,Scutellaria baicalensis,fructus forsythiae,Acacia catechu,Respectively.Result:1 A total of 130 strains of Clostridium difficile were selected in this study.The strains were mainly from Shijiazhuang,Xingtai and Cangzhou,The time was from 2010 to 2014,and the ratio of male to female was 70:60.Toxin A toxin B toxin positive binary toxin negative(tcd A+tcd B+cdt A-cdt B-)Clostridium difficile was 105 strains,toxin A toxin B toxin positive binary toxin positive(tcd A+tcd B+cdt A+cdt B+)Clostridium difficile was 3 strains,A toxin B toxin negative toxin binary toxin negative(tcd A-tcd B-cdt A-cdt B-)Clostridium difficile was 22 strains.130 strains of Clostridium difficile had a total of 36 PCR ribotyping,HB1 is the most common in all PCR ribotyping,a total of 23 strains,followed by HB5 type 18 strains,HB6 type 17 strains,the three accounted for a total of 44.6%.2 MIC0.086-0.249mg/ml for Chinese gallnut,MIC 4.128-22.423mg/ml for acacia catechu,The MIC was not found in scutellaria baicalensis and fructus forsythiae,Part of Coptis chinensis had antibacterial activity against CD.3 There was no difference in MIC of catechin PCR-ribotype epidemic typing and non epidemic typing,P=0.89.But the MIC of Galla chinensis has difference in epidemic typing and non epidemic typing,P=0.43.Conclusion:1 Galla chinensis,Coptis and catechu have antibacterial activity against Clostridium difficile,which is expected to be a potential therapeutic agent.2 Forsythia and Scutellaria has no antibacterial activity to Clostridium difficile.3 The MIC value of Chinese gallnut was lower than that of the non dominant strains in the dominant strain group,and the MIC value of catechu was not statistically different between the dominant strain group and the non dominant strain group.