The Protective Effects And Mechanism Of Rosamultin On The Acute Hypobaric Hypoxia Injury Of Bone Tissue In Rats

Objective: This study aimed to determine the research status of Monomer compounds rosamultin Extract from Potentill anserine L in Department of medicinal chemistry armed police Logistics College.To explore the pharmacological activities and mechanism of rosamultin against acute high altitude hypoxia bone injury,we established a rat model of acute high altitude hypoxia.Method: 60 male SD rats were randomly divided into 5 group(12 rats / group): sham group(C),acute hypoxia model group(M),high dose rosamultin group(15 mg/kg)(group H),low dose rosamultin group(5 mg/kg)(L),positive dose dexamethasone group(0.36 mg/kg)(D).To induce acute high altitude hypoxia bone injury,rats were placed an CNC low pressure chamber.Before hypoxia,a suitable dose rosamultin was been used.First,ELISA and enzyme detection methods determination of rat plasma and bone tissue homogenate of calcium(Ca),phosphorus(P),alkaline phosphatase(ALP)activity.View the morphological changes of rat bone tissue cells we used Hematoxylin eosin(HE)staining,ALP staining and Tartrate resistant acid phosphatase(TRAP)staining.Second,immunohistochemical staining evaluated changes in the levels of specific proteins in the bone(osteocalcin,matrix metalloproteinase-9(MMP-9),osteoprotegerin(OPG)and receptor activator of nuclear factor ? B ligand(RANKL)).And then,Using three point bending loading device of three point bending experiments on the mechanical properties of rats femur,the mechanical properties target include bending stress and bending strain,fracture displacement,and calculation of the elastic modulus of bone tissue of rats.Finally,using Micro-CT imaging technology to evaluation the bone volume fraction detection of rat femoral metaphysis the end of the bone tissue(BV/TV),bone mineral density(BMC),trabecular bone volume(Tb.N),trabecular separation(Tb.Sp)and bone microstructure index.Results: Rosamultin could significantly increase rats serum calcium concentration in plasma of acute high altitude hypoxia,reduce serum phosphate concentration in plasma,and significantly improve the bone tissue of acute hypoxia in rat and the activity of ALP,reduce bone tissue TRAP activity,increased the expression of osteocalcin and OPG protein in bone tissue,reduce the expression of MMP-9 and RANKL protein in bone tissue,the morphological changes of the cells to reduce the damage of acute hypoxia of bone trabecular bone,reduce acute hypoxia induced damage,increase in the number of trabecular bone,reducing bone trabecula separation,and improve the mechanical properties of bone tissue in acute hypobaric hypoxia in rats,improve bone strength and bone the toughness of rat bone tissue.Conclusion: In our conclusion,Monomer compounds rosamultin has an significant pharmacological activities against acute hypoxia injury,and these effects are associated with promotion bone tissue formed and inhibit bone tissue resorption in rat.These mechanism may be by thereby regulating bone tissue of the bone remodeling process,improve the structure of hypoxia and function of bone tissue,improve the mechanical properties of bone tissue,so as to exert pharmacological activity against hypoxia.