Investigation Of Ciprofloxacin On Autophagy Of Primary Hepatocytes

Background: The study found that autophagy plays an important role in the treatment of a variety of diseases,ciprofloxacin is lysosomotropic drug,lysosome is a key organelle in the process of autophagy,ciprofloxacin may affect the physiological or pathological function of hepatocytes through autophagy.Objective: To detect the autophagy by SNLYSO senor,screening differentially expressed autophagy related genes by mRNA expression.analysing the expression of autophagy-related genes,these results can provide theoretical and experimental techniques for diagnosis and treatment evidence of Ciprofloxacin.Methods: Swiss mice were housed in SPF system and divided into experimental group and control group.the experimental group were treated with ciprofloxacin and the control group was treated with normal saline 7 days,and the mouse primary hepatocytes were isolated by collagenase perfusion method.autophagy was observed by staining with SNLYSO senor and the whole gene expression was detected by Agilent genome microarray.Results: The experimental group and the control group showed autophagy bright spots,but the experimental group spots were smaller and less.Foldchange?1.5 or ?0.75,P <0.05,screening out the different expression of 458 genes,the expression of 133 genes were upregulated and 325 genes were down-regulated.Compared with 1971 autophagy-related genes from GENECARDS,the expression of 9 autophagy related genes was up-regulated and27 autophagy-related genes was down-regulated,KEGG showed 16 pathways,including Mismatch repair,Glycosaminoglycan,DNA replication,Mucin type O-glycan biosynthesis,Galactose metabolism,Nucleotide excision repair,homologous recombination,Homologous recombination,Glioma,p53 signaling pathway,Sphingolipid metabolism,Inositol phosphate metabolism,Hypertrophic cardiomyopathy,Protein digestion and absorption.Conclusion: The autophagy of primary hepatocytes is inhibited by ciprofloxacin,and ciprofloxacin can significantly affect the genes expression of primary hepatocytes.The distribution of the differential genes by the KEGG were signal transduction,glucose metabolism,further study is necessary to confirm it.