The Effects Of Bioactive Glass On The Proliferation And Osteogenic Differentiation Of L929 Cells In Vitro

Objectives To investigate the effects in vitro of bioactive glass on cell proliferation,cell cycle and osteogenic differentiation of mouse fibroblasts L929,and provide the basic experimental basis for the clinical application of bioactive glass for periodontal tissue regeneration.Methods The experimental study was divided into two parts: 1 Effects of bioactive glass on the proliferation of L929 cells.Prepared bioactive glass extract,which was diluted with RPMI1640 culture medium(supplemented with 10% NCS)into different concentrations of BG extract and used for culture L929 cells 24 h,48h and 72 h,the effects of different concentrations of bioactive glass extract on proliferation and cell cycle of L929 cells were detected by MTT assay and flow cytometry(FCM).2 Effects of bioactive Glass on the differentiation of L929 cells.After culture with 24 h,48h and 72 h,the effects of different concentrations of bioactive glass extract on the differentiation of L929 cells in vitro were investigated by alkaline phosphatase(ALP)activity assay.Results After 24 h,48h and 72 h,with culture time,MTT and alkaline phosphatase(ALP)method showed the OD values of L929 cultured with the 0.0625g/L~1.25g/L diluted extracts were significantly higher than that of the control,the difference was statistically significant(P<0.05),the OD value of 0.0625g/L diluted extract was most remarkable than control group(P<0.05);the OD value of 2.5g/L diluted extract showed slightly lower when compared with the control group,the difference was no statistically significant(P>0.05);The OD value of 5g/L diluted extract were significantly lower than that of the control,the difference was statistically significant(P<0.05).The results of Flow cytometry(FCM)were consistent with the above experimental results.The cell proliferation index(PI)scored highest in 0.0625g/L of BG extract concentration,and the PI value scored lowest in 5 g/L of BG extract concentration(P<0.05).Conclusions 1 Bioactive glass extract could stimulate proliferation and osteogenic differentiation of L929 cells in a certain concentration range.2 L929 cells cultured with lower concentration of BG extract enchanced proliferation and differentiation more obviously,while cultured with higher concentration of BG extract could inhibit L929 cells proliferation and differentiation.