Study On Preparation Technology Of CFTs And CTFTs And Its Mechanism Of Reducing Weight And Losing Weight

Because of the genetic causes,lifestyle or diet structure unreasonable,overweight and obese people is increasing,"lose weight" has also become a hot topic in the field of medicine,at the same time also brings the harm of obesity has aroused people's attention,but also the weight-loss drugs most weight-loss drugs emerge in an endless stream,because of security problems have been evacuated the city,the slimming medicine is very lack,weight-loss drug market is still very huge.Chitosan(chitosan,CTS)is a kind of natural carbohydrate products containing active free amino groups,has hypolipidemic,hypoglycemic activity,chitosan oligosaccharide(chitosan oligosaccharides COS)is one of the degradation products,biological activity and bioavailability were higher than that of chitosan.Previous studies confirmed CTS and COS have to lose weight the activity for the development of effective,stable and controllable,taking the foundation of modern preparation is convenient.Therefore,this paper on the basis of CTS in our research group,COS made easy to take tablets,for the development of new drugs of natural carbohydrate weight loss is significant.In this paper,the chitosan oligosaccharide with average molecular weight less than 1000Da(COS)made of chitosan film coated tablet(Chitosan oligosaccharide film coated tablets,CFTs,CTS)made of chitosan film coated tablet(Chitosan film coated tablets,CTFTs),to investigate the preparation method and content determination method.And through the establishment of animal model of obesity to lose weight,functional evaluation of CFTs and CTFTs,and the weight loss activity of CFTs and CTFTs were compared.The results show that under the condition of room temperature,humidity is less than 40%,the preparation of CTS experiment mainly through the study of screening and orthogonal design methods of different excipients between the influence of formulation factors and forming factors of chitosan tablet preparation process,the optimal prescription scheme as follows: 74% chitosan,5%PPVP,9%CaHPO4,10%MCC,2% powder silica gel,5%CMC-NA glue(water wetting agent)3% coating weight,the indicators according to the prescription made of CTFTs are consistent with the requirements of Chinese Pharmacopoeia;The preparation of COS experimental design method mainly through material screening and single factor explore the influence of formulation factors and forming factors of chitosan oligosaccharide tablet preparation process,the final formulation is: 87.5%,4%PPVP,4%CaHPO4,chitosan 4%MCC,0.5% magnesium stearate,5%PVP solution(95% ethanol),3% coating weight.The indicators according to the prescription made tablets are consistent with the requirements of Chinese Pharmacopoeia,and the preparation process is simple and suitable for industrial production.CFTs,CTFTs can effectively reduce the body weight of rats increased,and had no effect on food intake in obese rats,reduce the body fat of obese rats,and can significantly reduce the T-CHO content in serum of obese rats,TG and LDL-C,increased the content of HDL-C in serum,improve blood lipid levels,with lipid-lowering effect the.CFTs high dose group,middle dose of CFTs group increased weight had no significant difference with Orlistat,the effect of weight loss.CTFTs can also significantly reduce the rat body weight gain,does not affect the food intake,reduce fat / body ratio,suggesting a weight function better;can reduce the levels of T-CHO,TG and LDL-C content,increase the content of HDL-C,which had a significant dose of high dose of CTFTS group and CTFTS group(P < 0.05).It is suggested that dose can improve the blood lipid level of.CTFTS had no significant difference in weight gain with the inhibitory effects of Orlistat weight,weight loss.Weight loss function size: CFTs > CTFTs.The expression of FQRT-PCR in rat epididymal fat in the detection of early adipose differentiation factor c/EBP beta gamma mRNA and PPAR,the results showed that CFTS and CTFTS can inhibit the epididymal adipose tissue in c/EBP beta gamma mRNA,expression of PPAR,inhibit fat differentiation,reduce lipid accumulation.At the same time,also detected brown fat thermogenesis the rat markers UCP1,PRDM16 thermal control factor,the expression of DIO2 mRNA,the results show that CFTs can promote UCP1 in brown adipose tissue PRDM16 expression of DIO2,mRNA,increased heat production,energy consumption,and achieve the purpose of weight loss.