Expression And Function Of Long Non-coding RNA PTENP1 In Glioma

[Background]Glioma is the most common primary central nervous system tumors in adults.According to the statistics of the Central Brain Tumor Registry of the United States(CBTRUS),accounting for 28% of all central nervous system tumors,and 80% of malignant tumors.At present,the mainly treatment of glioma include surgery,radiotherapy and chemotherapy.Although in recent years rapidly advances in treatment,the average prognosis is still less than 15 months.At the same time,the adjuvant therapy like radiotherapy and chemotherapy may also produce side effects of central nervous system.Therefore,there is an urgent need to develop effective treatment for glioma patients.In recent years,the related genes,molecular mechanisms and signaling pathways about glioma formation and development have been extensively studied,however,the pathogenesis is still not clear.In order to improve the diagnosis and treatment of glioma,it is very significant to explore the genes related to occurrence and progression of human brain glioma.Long non-coding RNA(lnc RNA)is a kind of non-coding RNA with a length of more than 200 nucleotides.In recent years,an increasing number of studies have shown that lnc RNA plays an critical role in the occurrence and development of multiple tumors.PTENP1 is a pseudogenes of the PTEN(phosphatase and tension homology deletion on chromosome 10,PTEN)tumor suppressor gene,which shows extensive sequence similarity with PTEN.Yu et al found that lnc RNA PTENP1 served as competing endogenous RNA(ce RNA)for the regulation of PTEN expression by competing mi R21 to suppress renal clear cell carcinoman progression.Xiong et al found that PTENP1 can inhibited the proliferation and migration of hepatocarcinoma cells through the MAPK signaling pathway.However,its expression in glioma,its function in occurrence and development of glioma have not yet been investigated.The aim of this study is to nvestigate the expression of PTENP1 in glioma tissue and normal brain tissue,then,we upregulate the expression of PTENP1 in glioma cell lines and detect cell proliferation,apoptosis and migration.[Objective and methods]1.Quantitative Real-time RT-PCRwere used to detect the expression of lnc RNA PTENP1 in 11 fresh glioma tissue samples and 5 normal brain tissue samples.2.Up-regulated the expression of lnc RNA PTENP1 in glioma cell lines,cell proliferation was detected by the cell-counting kit-8(CCK-8)assay.Transwell assay and Wound-healing assay were employed to examine the invasion and migration of the glioma cells.[Results]1.The results of Real time RT-PCR showed PTENP1 expression level in glioma tissues was significantly down-regulated compared with normal brain tissues(P<0.05).2.The pc DNA-PTENP1 was transfected into human glioma cell line SHG44,U251 to upregulate the expression of PTENP1,the results of Real time RT-PCR showed PTENP1 expression was markedly elevated(P<0.05).3.After 24,48,72 h,the proliferation of SHG44 and U251 was determined by CCK8 assay.the result showed that the SHG44 and U251 cells in transfected groups grew significantly slower than the cells in control group(P<0.05).The Transwell assay showed significant cell invasion inhibition in transfected groups compared with the control group(P<0.05).Wound-healing assay showed that overexpression of PTENP1 in SHG-44 and U251 cells inhibited migration(P <0.05).[Conclusions]Our results suggest that lnc RNA PTENP1 may serve as a novel tumor suppressor gene in glioma,which can inhibit the proliferation,invasion and migration of glioma cells.Further research about lnc RNA PTENP1 may provide a novel biomarker and therapeutic target of glioma for cancer clinic in future.